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Over-expression of StAPX in tobacco improves seed germination and increases early seedling tolerance to salinity and osmotic stresses

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Abstract

Ascorbate peroxidase plays a key role in scavenging reactive oxygen species under environmental stresses and in protecting plant cells against toxic effects. The Solanum lycopersicum thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco under the control of the cauliflower mosaic virus 35S promoter. Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel blot analysis confirmed that StAPX was transferred into the tobacco genome and StAPX was induced by salt and osmotic stresses in tomato leaves. Over-expression of StAPX in tobacco improved seed germination rate and elevated stress tolerance during post-germination development. Two transgenic lines showed higher APX activity and accumulated less hydrogen peroxide than wild-type plants after stress treatments. The photosynthetic rates, the root lengths, the fresh and dry weights of the transgenic lines were distinctly higher than those of wild-type plants under stress conditions. Results indicated that the over-expression of StAPX had enhanced tolerance to salt stress and osmotic stress in transgenic tobacco plants.

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Acknowledgments

This research has been supported by the State Key Basic Research and Development Plan of China (2009CB118505), the Natural Science Foundation of China (30871458), Program for Changjiang Scholars and Innovative Research Team in University (Grant IRT0635) and the Startup Foundation of Advanced Professional in Jiangsu University (09JDG021).

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Authors and Affiliations

  1. College of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Wei-Hong Sun

  2. College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China

    Wei-Hong Sun, Ming Duan, De-Feng Shu, Sha Yang & Qing-Wei Meng

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  1. Wei-Hong Sun
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  2. Ming Duan
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  3. De-Feng Shu
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  4. Sha Yang
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  5. Qing-Wei Meng
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Correspondence to Qing-Wei Meng.

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Communicated by K. Chong.

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Sun, WH., Duan, M., Shu, DF. et al. Over-expression of StAPX in tobacco improves seed germination and increases early seedling tolerance to salinity and osmotic stresses. Plant Cell Rep 29, 917–926 (2010). https://doi.org/10.1007/s00299-010-0878-9

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  • DOI: https://doi.org/10.1007/s00299-010-0878-9

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