Abstract
Transcription mediated by RNA polymerase II depends on a set of different transcription factors to form the pre-initiation complex. TFIIA is involved in the construction of this complex and increases the affinity of TBP for the DNA union region in vitro. In this study, we characterized the ScTFIIAγ gene, which encodes a homolog of the smaller subunit (γ) of transcription factor TFIIA in sugarcane. RNA blot analysis showed that ScTFIIAγ transcripts accumulate in all tissues evaluated, with higher levels in leaf roll and flowers. In situ hybridization showed that ScTFIIAγ was expressed in different cells of the reproductive meristem. In sugarcane plantlets, methyl jasmonate and absicic acid treatments as well as phosphate starvation had no influence on ScTFIIAγ transcript accumulation. The subcelullar localization assay demonstrates that ScTFIIAγ protein is directed to the cell nucleus. The phylogenetic analysis, the expression in several tissues and under different treatments and the nuclear localization are in line with the putative role of ScTFIIAγ as a subunit of basal transcription factor.
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Acknowledgments
This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grants 01/07546-1 and 05/58104-0 to MM. We are very grateful to Eduardo Kiyota for technical assistance and Raphael S. Mattos for his help with the phylogenetic analysis. PLC, RGT and MGKB received fellowships from FAPESP.
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Communicated by P. Puigdomenech.
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Gentile, A., Da Cruz, P., Tavares, R.G. et al. Molecular characterization of ScTFIIAγ, encoding the putative TFIIA small subunit from sugarcane. Plant Cell Rep 29, 857–864 (2010). https://doi.org/10.1007/s00299-010-0871-3
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DOI: https://doi.org/10.1007/s00299-010-0871-3