A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes
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Crop adaptation to abiotic stresses requires alterations in expression of a large number of stress protection genes and their regulators, including transcription factors. In this study, the expression levels of ten MYB transcription factor genes from wheat (Triticum aestivum) were examined in two recombinant inbred lines contrasting in their salt tolerance in response to salt or drought stress. Quantitative RT-PCR analysis revealed that four MYB genes were consistently up-regulated in the seedling roots of both genotypes under short-term salt treatment. Three MYB genes were found to be up-regulated in both genotypes under long-term salt stress. One MYB gene was up-regulated in both genotypes under both short- and long-term salt stress. Of these salt up-regulated MYB genes, one MYB gene (TaMYBsdu1) was markedly up-regulated in the leaf and root of wheat under long-term drought stress. In addition, TaMYBsdu1 showed higher expression levels in the salt-tolerant genotype than in the susceptible genotype under salt stress. These data suggest that TaMYBsdu1 is a potentially important regulator involved in wheat adaptation to both salt and drought stresses.
KeywordsDrought tolerance Expression profiling MYB transcription factor Salt tolerance Wheat breeding
We wish to thank Krassen Dimitrov for financial support for part of this study, and Nasser Sewelam, Shazia Iram and other members of the Schenk laboratory for technical assistance. We would like to thank The University of Tehran for providing a doctoral fellowship to MR.
- Kam J, Gresshoff PM, Shorter R, Xue GP (2008) The Q-type C2H2 zinc finger subfamily of transcription factors in Triticum aestivum is predominantly expressed in roots and enriched with members containing an EAR repressor motif and responsive to drought stress. Plant Mol Biol 67:305–322CrossRefPubMedGoogle Scholar
- Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406CrossRefPubMedGoogle Scholar
- Pfaffl MW (2001) A mathematical model for relative quantification of real-time RT-PCR. Nucleic Acid Res 29:2002–2007Google Scholar
- Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498CrossRefPubMedGoogle Scholar
- Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinazachi K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathways under drought and high-salinity conditions. Proc Natl Acad Sci USA 97:11632–11637CrossRefPubMedGoogle Scholar