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RETRACTED ARTICLE: Transgenic ramie [Boehmeria nivea (L.) Gaud.]: factors affecting the efficiency of Agrobacterium tumefaciens-mediated transformation and regeneration

Plant Cell Reports volume 28, pages 1319–1327 (2009)Cite this article

This article was retracted on 23 September 2009

This article was retracted on 23 September 2009

Abstract

In the present study, an efficient Agrobacterium-mediated gene transformation system was developed for ramie [Boehmeria nivea (L.) Gaud.] based on the examinations of several factors affecting plant transformation efficiency. The effects of Agrobacterium cell density, acetosyringone, co-cultivation temperature, co-cultivation duration, co-cultivation photoperiod and pH on stable transformation were evaluated. Agrobacterium at a concentration of OD = 0.5–0.8 improved the efficiency of transformation. Concentration of acetosyringone at 50 mg/L during co-cultivation significantly increased transformation efficiency. Co-cultivation at 20°C, in comparison to 15, 25 and 28°C, consistently resulted in higher transformation frequencies. A relatively short co-cultivation duration (3 days) was optimal for ramie transformation. Co-cultivation medium at pH 5.9 and co-cultivation in darkness both improved the transformation efficiencies of ramie. An overall scheme for producing transgenic ramie is presented, through which an average transformation rate from 10.5 to 24.7% in five ramie varieties was obtained. Stable expression and integration of the transgenes were confirmed by histochemical GUS assay, kanamycin painting assay, PCR and Southern blotting. This optimized transformation system should be employed for efficient Agrobacterium-mediated transformation of ramie.

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Abbreviations

GUS :

β-Glucuronidase

IAA:

Indole-3-aceticacid

Km:

Kanamycin

MS:

Murashige and Skoog medium

NPTII:

Neomycin phosphotransferase

PCR:

Polymerase chain reaction

TDZ:

Thidiazuron

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Acknowledgments

We are grateful to Prof. Conghua Xie (College of Horticulture and Forestry Science, Huazhong Agricultural University) for providing plasmid and Agrobacterium strain. We also appreciate Prof. Xinalong Zhang and Dr. Shuangxia Jin for their helpful suggestions in this study. The author Bo Wang wishes to thank Prof. Jihong Liu and Prof. Atanas Atanassov for their critical reading of this manuscript. This work was supported by grants from the National Natural Science Foundation of China (no. 30800696), the Ph.D. Programs Foundation of Ministry of Education of China (no. 20070504059) and R&D Special Fund for Public Welfare Industry of China.

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Affiliations

  1. College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, Hubei, China

    Bo Wang, Lijun Liu, Xuxia Wang, Jinyu Yang, Zhenxia Sun, Na Zhang, Shimei Gao, Xiulong Xing & Dingxiang Peng

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  1. Bo Wang
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  2. Lijun Liu
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  3. Xuxia Wang
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  8. Xiulong Xing
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  9. Dingxiang Peng
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Correspondence to Dingxiang Peng.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-009-0773-4

Communicated by R. Rose.

This article has been retracted because part of the data shown has already been published before, by different authors.

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Wang, B., Liu, L., Wang, X. et al. RETRACTED ARTICLE: Transgenic ramie [Boehmeria nivea (L.) Gaud.]: factors affecting the efficiency of Agrobacterium tumefaciens-mediated transformation and regeneration. Plant Cell Rep 28, 1319–1327 (2009). https://doi.org/10.1007/s00299-009-0732-0

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Keywords

  • Ramie
  • GUS
  • Cotyledon
  • Organogenesis
  • Transformation