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Molecular analyses of the rice glutamate dehydrogenase gene family and their response to nitrogen and phosphorous deprivation

  • Physiology and Biochemistry
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Abstract

Glutamate dehydrogenases (GDH, EC 1.4.1.2~4) are ubiquitous enzymes encoded by GDH genes. So far, at least two GDH members have been characterized in plants, but most members of this family in rice remains to be characterized. Here, we show that four putative GDH genes (OsGDH1-4) are present in the rice genome. The GDH sequences from rice and other species can be classified into two types (I and II). OsGDH1-3 belonged to type II genes, whereas OsGDH4 belonged to type I like gene. Our data implied that the expansion rate of type I genes was much slower than that of type II genes and species-specific expansion contributed to the evolution of type II genes in plants. The expression levels of the different members of GDH family in rice were evaluated using quantitative real-time PCR and microarray analysis. Gene expression patterns revealed that OsGDH1, OsGDH2, and OsGDH4 are expressed ubiquitously in various tissues, whereas OsGDH3 expression is glumes and stamens specific. The expression of the OsGDH family members responded differentially to nitrogen and phosphorus-deprivation, indicating their roles under such stress conditions. Implications of the expression patterns with respect to the functions of these genes were discussed.

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Abbreviations

GDH:

Glutamate dehydrogenase

Glu:

Glutamate

GOGAT:

Glutamate synthase

GS:

Glutamine synthetase

HCA:

Hierarchical cluster analysis

N− :

Nitrogen depriving or nitrogen-free solution

P− :

Phosphorus depriving or phosphorus-free solution

qPCR:

Quantitative real-time polymerase chain reaction

α-KG:

α-Ketoglutarate

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Acknowledgments

This research was supported in part by grants from the National Basic Research Program of China (2005CB120905), the National Special Key Project of China on Functional Genomics of Major Plants and Animals, the National Natural Science Foundation of China, and the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China (No. 707045).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China

    Xuhua Qiu, Weibo Xie, Xingming Lian & Qifa Zhang

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  1. Xuhua Qiu
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  2. Weibo Xie
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  3. Xingming Lian
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  4. Qifa Zhang
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Correspondence to Xingming Lian.

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Communicated by Y. Lu.

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Qiu, X., Xie, W., Lian, X. et al. Molecular analyses of the rice glutamate dehydrogenase gene family and their response to nitrogen and phosphorous deprivation. Plant Cell Rep 28, 1115–1126 (2009). https://doi.org/10.1007/s00299-009-0709-z

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