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Circadian rhythm of (Z)- and (E)-2-β-d-glucopyranosyloxy-4-methoxy cinnamic acids and herniarin in leaves of Matricaria chamomilla

  • Biotic and Abiotic Stress
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Abstract

Chamomile (Matricaria chamomilla) in the above-ground organs synthesizes and accumulates (Z)- and (E)-2-β-d-glucopyranosyloxy-4-methoxy cinnamic acids (GMCA), the precursors of phytoanticipin herniarin (7-methoxycoumarin). The diurnal rhythmicity of the sum of GMCA (maximum before daybreak) and herniarin (acrophase at 10 h 21 min of circadian time) was observed under artificial lighting conditions LD 12:12. The acrophase is the time point of the maximum of the sinusoidal curve fitted to the experimental data. In continuous light, the circadian rhythms of both compounds were first described with similar acrophases of endogenous rhythms; a significantly different result from that in synchronized conditions. The rhythms’ mesor (the mean value of the sinusoidal curve fitted to the experimental data) under free-running conditions was not influenced. Abiotic stress under synchronized conditions decreased the average content of GMCA to half of the original level and eliminated the rhythmicity. In contrast, the rhythm of herniarin continued, though its content significantly increased. Nitrogen deficiency resulted in a significant increase in GMCA content, which did not manifest any rhythmicity while the rhythm of herniarin continued. Circadian control of herniarin could be considered as a component of the plant’s specialized defence mechanisms.

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Abbreviations

GMCA:

(Z)- and (E)-2-β-d-glucopyranosyloxy-4-methoxy cinnamic acids

LD:

Light–dark

LL:

Continuous light

MDA:

Malondialdehyde

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Acknowledgment

This work was supported by the grant agency VEGA (project no. 1/0444/03). We thank Mrs. Anna Michalčová and Mrs. Margita Buzinkaiová for their valuable technical assistance.

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Authors and Affiliations

  1. Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 041 67, Kosice, Slovak Republic

    Miroslav Repčák, Beňadik Šmajda, Jozef Kováčik & Adriana Eliašová

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  1. Miroslav Repčák
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  2. Beňadik Šmajda
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  3. Jozef Kováčik
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  4. Adriana Eliašová
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Correspondence to Miroslav Repčák.

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Communicated by M. Petersen.

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Repčák, M., Šmajda, B., Kováčik, J. et al. Circadian rhythm of (Z)- and (E)-2-β-d-glucopyranosyloxy-4-methoxy cinnamic acids and herniarin in leaves of Matricaria chamomilla . Plant Cell Rep 28, 1137–1143 (2009). https://doi.org/10.1007/s00299-009-0708-0

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  • DOI: https://doi.org/10.1007/s00299-009-0708-0

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