Plant Cell Reports

, Volume 28, Issue 7, pp 1095–1104 | Cite as

A transformation booster sequence (TBS) from Petunia hybrida functions as an enhancer-blocking insulator in Arabidopsis thaliana

  • Jean-Michel Hily
  • Stacy D. Singer
  • Yazhou Yang
  • Zongrang Liu
Genetics and Genomics

Abstract

Several matrix-attachment regions (MARs) from animals have been shown to block interactions between an enhancer and promoter when situated between the two. Since a similar function for plant MARs has not been discerned, we tested the Zea maysADH1 5′ MAR, Nicotiana tabacumRb7 3′ MAR and a transformation booster sequence (TBS) MAR from Petunia hybrida for their ability to impede enhancer–promoter interactions in Arabidopsis thaliana. Stable transgenic lines containing vectors in which one of the three MAR elements or a 4 kb control sequence were interposed between the cauliflower mosaic virus35S enhancer and a flower-specific AGAMOUS second intron-derived promoter (AGIP)::β-glucuronidase (GUS) fusion were assayed for GUS expression in vegetative tissues. We demonstrate that the TBS MAR element, but not the ADH1 or Rb7 MARs, is able to block interactions between the 35S enhancer and AGIP without compromising the function of either with elements from which they are not insulated.

Keywords

MAR Transformation booster sequence 35S promoter/enhancer AGAMOUS second intron Arabidopsis thaliana Enhancer-blocking insulator 

Abbreviations

MAR

Matrix attachment region

TBS

Transformation booster sequence

GUS

β-Glucuronidase

AGIP

AGAMOUS second intron-derived promoter

35S

35S cauliflower mosaic virus promoter/enhancer

GFP

Green fluorescent protein

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jean-Michel Hily
    • 1
    • 2
  • Stacy D. Singer
    • 1
  • Yazhou Yang
    • 1
  • Zongrang Liu
    • 1
  1. 1.USDA-ARS Appalachian Fruit Research StationKearneysvilleUSA
  2. 2.Department of Plant Pathology, New York State Agricultural Experiment StationCornell UniversityGenevaUSA

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