Abstract
Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs.
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Abbreviations
- BAP:
-
6-Benzylaminopurine
- DAPI:
-
4′-6-Diamidino-2-phenylindole
- 2, 4-D:
-
2, 4-Dichlorophenoxyacetic acid
- GA3 :
-
Gibberellic acid
- SSR:
-
Simple sequence repeats
- WBS:
-
Weeks before splitting
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Acknowledgments
We gratefully acknowledge Prof. R. Pathirana and Dr. R. Bicknell, (Crop and Food Research, New Zealand) for their assistance in flow-cytometry. We are also thankful to Dr. C. Bandaranayake, C. Perera and Mrs Sandhya Fernando (Genetics and Plant Breeding Division, Coconut Research Institute, Sri Lanka) for the assistance in SSR marker analysis.
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Perera, P.I.P., Perera, L., Hocher, V. et al. Use of SSR markers to determine the anther-derived homozygous lines in coconut. Plant Cell Rep 27, 1697–1703 (2008). https://doi.org/10.1007/s00299-008-0592-z
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DOI: https://doi.org/10.1007/s00299-008-0592-z