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Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate

  • Cell Biology and Morphogenesis
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Abstract

The Arabidopsis genome has six families of dynamin-related proteins. One of these families includes DRP2A and DRP2B. The domain structures of proteins of this family are most similar to those of the animal endocytosis protein, dynamin. In this study, the signals of GFP-tagged DRP2B were strongly detected in the cell plate of Arabidopsis root tip cells and tobacco cultured cells. Time-lapse observations of these signals during cytokinesis in tobacco cultured cells suggested that DRP2B mainly localized to the newly formed part of the cell plate, and that the localization dynamics of DRP2B was quite similar to that of DRP1A, which is an Arabidopsis dynamin-related protein that is closely related to soybean phragmoplastin. These results indicate that Arabidopsis dynamin-related proteins, DRP1A and DRP2B, from two different families, participate in membrane remodeling at a similar place in the cell plate.

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Abbreviations

DRP:

Dynamin-related protein

GFP:

Green fluorescent protein

RFP:

Red fluorescent protein

BY-2:

Bright yellow-2

CLSM:

Confocal laser scanning microscopy

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Acknowledgments

This research was supported by grants-in-aid for scientific research from the Japan Society for the Promotion of Science and grants-in-aid to M. Fujimoto for Scientific Research for Plant Graduate Student from Nara Institute of Science and Technology, supported by The Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Masaru Fujimoto, Shin-ichi Arimura, Mikio Nakazono & Nobuhiro Tsutsumi

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  1. Masaru Fujimoto
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  2. Shin-ichi Arimura
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  3. Mikio Nakazono
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  4. Nobuhiro Tsutsumi
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Corresponding author

Correspondence to Nobuhiro Tsutsumi.

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Communicated by F. Brandizzi.

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Fujimoto, M., Arimura, Si., Nakazono, M. et al. Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate. Plant Cell Rep 27, 1581–1586 (2008). https://doi.org/10.1007/s00299-008-0583-0

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  • DOI: https://doi.org/10.1007/s00299-008-0583-0

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