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Cadmium induced oxidative stress and changes in soluble and ionically bound cell wall peroxidase activities in roots of seedling and 3–4 leaf stage plants of Brassica juncea (L.) czern

  • Biotic and Abiotic Stress
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Abstract

Metabolic adaptations to heavy metal toxicity in plants are thought to be related with developmental growth stage and the type of metal by which plant is affected. In the present study, changes in ionically bound CWP, soluble peroxidase activity, H2O2 level and Malonaldehyde content in roots of cadmium and copper stressed seedlings and cadmium stressed 3–4 leaf stage plants of Brassica juncea were investigated. Cadmium inhibits root growth and reduces fresh biomass. The reduction in root growth and fresh biomass is correlated with increased lipid peroxidation and reduced tolerance. Treatment with cadmium resulted in an increase in ionically bound CWP activity in roots of seedlings but no significant change in its activity was found in roots of 3–4 leaf stage plants. Increased level of H2O2 in roots of cadmium and copper treated seedlings, show a direct correlation with increased activity of ionically bound CWP. H2O2 level in 3–4 leaf stage plant roots was found to be very low. Soluble peroxidase activity decreased in cadmium (50 and 100 μM) treated seedlings but it was ineffective to cause any change in its activity in 3–4 leaf stage plants. Copper treated seedlings showed an increase in ionically bound CWP activity, H2O2 level and MDA content. Ascorbic acid (50 mM) pretreated seedlings shows significant decrease in ionically bound CWP activity when exposed to 50 μM cadmium. Hence, it is concluded that inhibition of root growth in Brassica juncea seedlings by cadmium, is associated with CWP catalyzed H2O2 dependent reactions which are involved in metabolic adaptations to heavy-metal stress.

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Abbreviations

CWP:

Cell wall peroxidase

MDA:

Malonaldehyde

ROS:

Reactive oxygen species

H2O2 :

Hydrogen peroxide

TMB:

3, 3′, 5, 5′-tetramethyl benzidine

OFW:

Original fresh weight

FW:

Fresh weight

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Acknowledgments

G. S. Shekhawat is thankful to Professor Aditya Shastri, Vice chancellor, Banasthali University, Rajasthan, India for kind cooperation.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India

    Kusum Verma, G. S. Shekhawat, Astha Sharma & V. Sharma

  2. Department of Botany, Mizoram University, Aizawl, 796001, Mizoram, India

    S. K. Mehta

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  1. Kusum Verma
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  2. G. S. Shekhawat
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  3. Astha Sharma
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  4. S. K. Mehta
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Correspondence to G. S. Shekhawat.

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Communicated by J. Zou.

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Verma, K., Shekhawat, G.S., Sharma, A. et al. Cadmium induced oxidative stress and changes in soluble and ionically bound cell wall peroxidase activities in roots of seedling and 3–4 leaf stage plants of Brassica juncea (L.) czern. Plant Cell Rep 27, 1261–1269 (2008). https://doi.org/10.1007/s00299-008-0552-7

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  • DOI: https://doi.org/10.1007/s00299-008-0552-7

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