Abstract
Notocactus scopa cv. Soonjung was subjected to in planta Agrobacterium tumefaciens-mediated transformation with vacuum infiltration, pin-pricking, and a combination of the two methods. The pin-pricking combined with vacuum infiltration (20–30 cmHg for 15 min) resulted in a transformation efficiency of 67–100%, and the expression of the uidA and nptII genes was detected in transformed cactus. The established in planta transformation technique generated a transgenic cactus with higher transformation efficiency, shortened selection process, and stable gene expression via asexual reproduction. All of the results showed that the in planta transformation method utilized in the current study provided an efficient and time-saving procedure for the delivery of genes into the cactus genome, and that this technique can be applied to other asexually reproducing succulent plant species.
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Abbreviations
- GUS:
-
β-Glucuronidase
- nptII :
-
Neomycin phoshotransferase
- X-Gluc:
-
5-Bromo-4-chloro-3-indolyl-β-d-glucuronic acid
- YEP:
-
Yeast extract peptone
- MS:
-
Murashige and Skoog media
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Acknowledgments
This research was supported by grants from the Agricultural Research and Development Promotion Center (ARPC) funded by the Ministry of Science and Technology of the Korean government.
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Communicated by J.R. Liu.
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Seol, E., Jung, Y., Lee, J. et al. In planta transformation of Notocactus scopa cv. Soonjung by Agrobacterium tumefaciens . Plant Cell Rep 27, 1197–1206 (2008). https://doi.org/10.1007/s00299-008-0540-y
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DOI: https://doi.org/10.1007/s00299-008-0540-y