Abstract
Although mungbean (Vigna radiata (L.) Wilczek) is commonly used as human food; the genomic resources of this species available in databases are limited. This study aims to develop expressed sequence tag (EST) resources for mungbean genes informative to early seedling development and chilling response. Two mungbean varieties that differ in disease resistance were found to also differ in their susceptibility to chilling temperatures. A total of 1,198 ESTs were obtained from one cDNA library and four PCR-select cDNA subtraction libraries; among these 523 were clustered into 136 contigs and 675 were singletons. The 811 non-redundant uniESTs were compared to GenBank using the Basic Local Alignment Search Tool (BLAST) and WU-BLAST algorithms, of these only 489 uniESTs had significant sequence homology, which may be involved in resuming the metabolic activity of seedlings, switching on photomorphogenesis, fuelling photosynthesis and/or initiating the unique developmental programs. Their encoded proteins may associate with regulatory proteins to trigger a direct stress response or participate in acclimation to environmental stressors. The uniEST platform reported will enrich the genomic resources of mungbean for functional genomic research on seedling development and chilling response of tropical crops and provide targets for improving the chilling tolerance of the tropical crops.
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Abbreviations
- ABA:
-
Abscisic acid
- ACC:
-
1-aminocyclopropane-1-carboxylate
- AVRDC:
-
Asian Vegetable Research and Development Center—The World Vegetable Center
- BLAST:
-
Basic Local Alignment Search Tool
- CLS:
-
Cercospora leaf spot
- CTAB:
-
Hexadecyltrimethylammonium bromide
- dai:
-
Day after imbibition
- DEPC:
-
Diethylpyrocarbonate
- EIN2:
-
Ethylene insensitive 2
- EST:
-
Expressed sequence tag
- GPI:
-
Glycosylphosphatidylinositol
- HSP:
-
Heat shock protein
- LEA:
-
Late embryogenesis abundant
- LTP:
-
Lipid transfer protein
- MYMV:
-
Mungbean yellow mosaic virus
- NCBI:
-
National Center for Biotechnology Information
- PDF:
-
Plant defensin
- PVP:
-
Polyvinylpyrrolidinone
- SAM:
-
S-adenosylmethionine
- TAIR:
-
The Arabidopsis Information Resource
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Acknowledgements
We thank Dr. C.C. Chen for insightful discussion. This work was supported by National Science Council grants NSC 94-2317-B-007-001.
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Communicated by D. Somers.
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Chen, LR., Markhart, A.H., Shanmugasundaram, S. et al. Early developmental and stress responsive ESTs from mungbean, Vigna radiata (L.) Wilczek, seedlings. Plant Cell Rep 27, 535–552 (2008). https://doi.org/10.1007/s00299-007-0488-3
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DOI: https://doi.org/10.1007/s00299-007-0488-3