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Enhancement of phenylethanoid glycosides biosynthesis in cell cultures of Cistanche deserticola by osmotic stress

  • Physiology and Biochemistry
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Abstract

The effect of osmotic stress on cell growth and phenylethanoid glycosides (PeGs) biosynthesis was investigated in cell suspension cultures of Cistanche deserticola Y. C. Ma, a desert medicinal plant grown in west region of China. Various initial sucrose concentrations significantly affected cell growth and PeGs biosynthesis in the suspension cultures, and the highest dry weight and PeGs accumulation reached 15.9 g l−1-DW and 20.7 mg g−1-DW respectively at the initial osmotic stress of 300 mOsm kg−1 where the sucrose concentration was 175.3 mM. Stoichiometric analysis with different combinations of sucrose and non-metabolic sugar (mannitol) or non-sugar osmotic agents (PEG and NaCl) revealed that osmotic stress itself was an important factor for enhancing PeGs biosynthesis in cell suspension cultures of C. deserticola. The maximum PeGs contents of 26.9 and 23.8 mg g−1-DW were obtained after 21 days at the combinations of 87.6 mM sucrose with 164.7 mM mannitol (303 mOsm kg−1) or 20 mM PEG respectively, which was higher than that of C. deserticola cell cultures grown under an initial sucrose concentration of 175.3 mM after 30 days. The stimulated PeGs accumulation in the cell suspension cultures was correlated to the increase of phenylalanine ammonium lyase (PAL) activity induced by osmotic stress.

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References

  • Cheng XY, Wei T, Guo B, Ni W, Liu CZ (2005a) Cistanche deserticola cell suspension cultures:Phenylethanoid glycosides biosynthesis and antioxidant activity. Process Biochem 40:3119–3124

    Article  CAS  Google Scholar 

  • Cheng XY, Guo B, Zhou HY, Ni W, Liu CZ (2005b) Repeated elicitation enhances phenylethanoid glycosides accumulation in cell suspension cultures of Cistanche deserticola. Biochem Eng J 24:203–207

    Article  CAS  Google Scholar 

  • Cheng XY, Zhou HY, Cui X, Ni W, Liu CZ (2006) Improvement of phenylethanoid glycosides biosynthesis in Cistanche deserticola cell suspension cultures by chitosan elicitor. J Biotech 121:253–260

    Article  CAS  Google Scholar 

  • Du NS, Liu JL (1993a) Determination of phenylethnoid glycosides in Cistanche deserticola by macrorecicular resin spectrophotometry. Nat Prod Res Dev 5:30–33

    CAS  Google Scholar 

  • Du NS, Wang H, Yi YH (1993b) Isolation and identification of phenylethnoid glycosides from Cistanche deserticola. Nat Prod Res Dev 5:5–8

    CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK (1956) Colorimetric methods for the determination of sugar and related substances. Anal Chem 28:350–357

    Article  CAS  Google Scholar 

  • Godoy-Hernandez GC, Vazquez-Flota FA, Loyola-Vargas VM (2000) The exposure to trans-cinnamic acid of osmotically stressed Catharanthus roseus cells cultured in a 14-l bioreactor increases alkaloid accumulation. Biotechnol Lett 22:921–925

    Article  CAS  Google Scholar 

  • Hahlbrock K, Grisebach H (1979) Enzymic controls in the biosynthesis of ligin and flavonoids. Ann Rev Plant Physiol 30:105–130

    Article  CAS  Google Scholar 

  • Kim SI, Choi HK, Kim JH (2001) Effect of osmotic pressure on paclitaxel production in suspension cell cultures of Taxus chinensis. Enzyme Microb Tech 28:202–209

    Article  CAS  Google Scholar 

  • Koukol J, Conn EE (1961) The metabolism of aromatic and properties of the phenylalanine deaminase of Hordeum vulgare. J Biol Chem 236:2692–2698

    PubMed  CAS  Google Scholar 

  • Lu MC (1998) Studies on the sedative effect of Cistanche deserticola. J Ethnopharmacol 59:161–165

    Article  PubMed  CAS  Google Scholar 

  • Lu CT, Mei XG (2003) Improvement of phenylethanoid glycosides production by a fungal elicitor in cell suspension culture of Cistanche deserticola. Biotechnol Lett 25:1437–1439

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497

    Article  CAS  Google Scholar 

  • Ouyang J, Wang XD, Zhao B, Yuan XF, Wang YC (2003) Effect of rare earth elements on the growth of Cistanche deserticola cells and the production of phenylethanoid glycosides. J Biotechnol 102:129–134

    Article  PubMed  CAS  Google Scholar 

  • Shohael AM, Hakrabarty D, Ali MB, Yu KW, Hahn EJ, Lee HL, Paek KY (2006) Enhancement of eleutherosides production in embryogenic cultures of Eleutherococcus sessiliflorus in response to sucrose-induced osmotic stress. Process Biochem 41:512–518

    Article  CAS  Google Scholar 

  • Smith JL, Smart NJ, Kurd WGW, Misawa M (1987) The use of organic and inorganic compounds to increase the accumulation of indole alkaloids in Catharanthus roseus (L.) Don cell suspension cultures. J Exp Bot 38:1507–1511

    Article  Google Scholar 

  • Steponkus PL, Lanphear FO (1967) Refinement of the triphenyl tetrazolium chloride method of determining cold injury. Plant Physiol 42:1423–1426

    Article  PubMed  CAS  Google Scholar 

  • Wang HQ, Wu JT, Zhong JJ (1999) Significant improvement of taxane production in suspension cultures of Taxus chinensis by sucrose feeding strategy. Process Biochem 35:479–483

    Article  Google Scholar 

  • Wang XW, Jiang XY, Wu LY, Wang XF (2001) Scavenging effect of glycosides of Cistanche deserticola on free radicals and its protection against OH-induced DNA damage in vitro. Chin Pharm J 36:29–31

    CAS  Google Scholar 

  • Wu JY, Wong K, Ho KP, Zhou LG (2005) Enhancement of saponin production in Panax ginseng cell culture by osmotic stress and nutrient feeding. Enzyme Microb Technol 36:133–138

    Article  CAS  Google Scholar 

  • Zhang YH, Zhong JJ, Yu JT (1995) Effect of osmotic pressure on cell growth and production of Ginseng saponin and polysaccharide in suspension cultures of Panax Notoginseng. Biotechnol Lett 17:1347–1350

    CAS  Google Scholar 

  • Zong GZ, He W, Wu GL, Chen LH (1996) Comparisons between Cistanche deserticola Y. C. Ma and Cistanche tubulisa (Scheak) Wight an some pharmacological activities. Tradit Chin Med J 21:436–438

    CAS  Google Scholar 

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Acknowledgments

The authors acknowledge the financial support from Innovation Research Program of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China

    Chun-Zhao Liu & Xi-Yu Cheng

  2. Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR, 72467, USA

    Chun-Zhao Liu

  3. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China

    Xi-Yu Cheng

Authors
  1. Chun-Zhao Liu
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  2. Xi-Yu Cheng
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Correspondence to Chun-Zhao Liu.

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Communicated by K. Kamo.

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Liu, CZ., Cheng, XY. Enhancement of phenylethanoid glycosides biosynthesis in cell cultures of Cistanche deserticola by osmotic stress. Plant Cell Rep 27, 357–362 (2008). https://doi.org/10.1007/s00299-007-0443-3

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  • DOI: https://doi.org/10.1007/s00299-007-0443-3

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