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Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture

  • Cell Biology and Morphogenesis
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Abstract

We report high frequencies of embryo production and plant regeneration through isolated microspore culture of hot pepper (Capsicum annuum L.). Microspores cultured in modified NLN medium (NLNS) divided and developed to embryos. Globular and heart-shaped embryos were observed from 3 weeks after the beginning of culture, and many embryos reached the cotyledonary stage after 4 weeks of culture. These cotyledonary embryos developed to plantlets after transfer to solid B5 basal medium. We also optimized conditions for embryo production by varying the pretreatment media, the carbon sources, and culture densities. Heat shock treatment in sucrose-starvation medium was more effective than in B5 medium. Direct comparisons of sucrose and maltose as carbon sources clearly demonstrated the superiority of sucrose compared to maltose, with the highest frequency of embryo production being obtained in 9% (w/v) sucrose. Microspore plating density was critical for efficient embryonic induction and development, with an optimal plating density of 8 × 104–10 × 104/ml. Under our optimized culture conditions, we obtained over 54 embryos, and an average of 5.5 cotyledonary embryos when 10 × 104 microspores were grown on an individual plate.

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Acknowledgments

This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by Korean government (MOST) (No. R01-2005-000-10338-0).

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Authors and Affiliations

  1. Department of Life Sciences, Mokwon University, 800 Doan-dong Seo-gu, Taejon, 302-729, South Korea

    Moonza Kim, In-Chang Jang, Jin-Ae Kim, Eun-Joon Park & Michung Yoon

  2. Department of Technology Marketing, Mokwon University, Taejon, South Korea

    Youngwon Lee

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  1. Moonza Kim
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  2. In-Chang Jang
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  3. Jin-Ae Kim
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  4. Eun-Joon Park
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Correspondence to Moonza Kim.

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Communicated by J.R. Liu.

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Kim, M., Jang, IC., Kim, JA. et al. Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Rep 27, 425–434 (2008). https://doi.org/10.1007/s00299-007-0442-4

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  • DOI: https://doi.org/10.1007/s00299-007-0442-4

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