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Authentic seed-specific activity of the Perilla oleosin 19 gene promoter in transgenic Arabidopsis

Plant Cell Reports volume 27, pages 29–37 (2008)Cite this article

Abstract

The Perilla (Perilla frutescens L. cv. Okdong) oleosin gene, PfOle19, produces a 19-kDa protein that is highly expressed only in seeds. The activity of the −2,015 bp 5′-upstream promoter region of this gene was investigated in transgenic Arabidopsis plants using the fusion reporter constructs of enhanced green fluorescent protein (EGFP) and β-glucuronidase (GUS). The PfOle19 promoter directs Egfp expression in developing siliques, but not in leaves, stems or roots. In the transgenic Arabidopsis, EGFP fluorescence and histochemical GUS staining were restricted to early seedlings, indehiscent siliques and mature seeds. Progressive 5′-deletions up to the −963 bp position of the PfOle19 promoter increases the spatial control of the gene expression in seeds, but reduces its quantitative levels of expression. Moreover, the activity of the PfOle19 promoter in mature seeds is 4- and 5-fold greater than that of the cauliflower mosaic virus 35S promoter in terms of both EGFP intensity and fluorometric GUS activity, respectively.

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Acknowledgments

This work was done by a Dual Degree Program between Sungkyunkwan University and Rural Development Administration (to K.-J. Chung) and financially supported by Rural Development Administration through BioGreen21 program (grant to S.-H. Ha), National Institute of Agricultural Biotechnology (NIAB 06-2-11-10-2) and the Ministry of Science and Technology through the Crop Functional Genomics Center (CG2141) in Korea.

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Affiliations

  1. National Institute of Agricultural Biotechnology, RDA, 225 Seodun-dong, Suwon, 441-707, South Korea

    Kyoung-Ji Chung, Bum-Soo Hahn, Kyung-Hwan Kim, Jong-Bum Kim, Yong-Hwan Kim & Sun-Hwa Ha

  2. Department of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746, South Korea

    Kyoung-Ji Chung & Joo-Sung Yang

  3. Institute of Biological Chemistry, Washington State University, Pullman, WA, 99164, USA

    Seon-Kap Hwang

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  1. Kyoung-Ji Chung
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  2. Seon-Kap Hwang
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  3. Bum-Soo Hahn
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  4. Kyung-Hwan Kim
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  5. Jong-Bum Kim
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Correspondence to Sun-Hwa Ha.

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Communicated by J.R. Liu.

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Chung, KJ., Hwang, SK., Hahn, BS. et al. Authentic seed-specific activity of the Perilla oleosin 19 gene promoter in transgenic Arabidopsis . Plant Cell Rep 27, 29–37 (2008). https://doi.org/10.1007/s00299-007-0440-6

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  • DOI: https://doi.org/10.1007/s00299-007-0440-6

Keywords

  • Arabidopsis
  • Oleosin
  • Perilla (Perilla frutescens L.)
  • Promoter
  • Seed-specific