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Cytological events in explants of Arabidopsis thaliana during early callogenesis

  • Cell Biology and Morphogenesis
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Abstract

Leaf explants of diploid (2n = 2x = 10) and autotetraploid (2n = 4x = 20) plants of Arabidopsis thaliana ecotype Columbia were cytologically and cytogenetically analysed to determine the time and the mechanisms of the process of polyploidization. The first polyploid cells were observed after the third day of culture in both genotypes of explants. Polyploid cells were the result of pre-existing mixoploidy in explants of A. thaliana. Other factors such as endoreduplication, endomitosis, abnormal microtubules arrangement and DNA damage may have induced polyploidization during early stages of callogenesis.

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Acknowledgments

This work was supported by grant No. 2PO4C 088 28 from the State Committee for Scientific Researches.

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Authors and Affiliations

  1. Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland

    A. Fras, J. Juchimiuk, D. Siwinska & J. Maluszynska

Authors
  1. A. Fras
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  2. J. Juchimiuk
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  3. D. Siwinska
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  4. J. Maluszynska
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Corresponding author

Correspondence to A. Fras.

Additional information

Communicated by P. Kumar.

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Fras, A., Juchimiuk, J., Siwinska, D. et al. Cytological events in explants of Arabidopsis thaliana during early callogenesis. Plant Cell Rep 26, 1933–1939 (2007). https://doi.org/10.1007/s00299-007-0415-7

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  • DOI: https://doi.org/10.1007/s00299-007-0415-7

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