Abstract
Homeobox genes are essential regulators of plant development. ATHB23, a class I homeodomain leucine zipper gene of Arabidopsis, was found to be induced by treatment with the phytohormone gibberellin (GA). In order to clarify its role in development, we performed a histochemical analysis of transgenic plants containing a construct with a GUS::GFP reporter under the control of the 1.5 kb upstream region of ATHB23. The construct was mainly expressed in young leaves and the styles of flowers but not in mature leaves. Microscopic examination of young leaves revealed that it was expressed in the adaxial domain of leaf primordia and the rib meristem. Expression of ATHB23, like that of GA5 encoding GA 20-oxidase, was reduced in mutants related to adaxial–abaxial leaf polarity (phb-1d, se-2, and kan1 kan2). Reduced expression of the GUS::GFP reporter gene was also observed in an se-2 background. These results indicate that ATHB23 is under the control of GA and other activators such as PHB, and is involved in establishing polarity during leaf development.
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References
Allen S, Detlef W, Martin FY (1999) The Arabidopsis thaliana MERISTEM LAYER1 promoter specifies epidermal expression in meristems and young primordial. Plant J 20:259–263
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
Eshed Y, Baum SF, Perea JV, Bowman JL (2001) Establishment of polarity in lateral organs of plants. Curr Biol 11:1251–1260
Fleet CM, Sun T (2005) A DELLAcate balance: the role of gibberellin in plant morphogenesis. Curr Opin Plant Biol 8:77–85
Garber RL, Kuroiwa A, Gehring WJ (1983) Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila. EMBO J 2:2027–2036
Grigg SP, Claudia C, Hay A, Tsiantis M (2005) SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis. Nature 437:1022–1026
Hanson J, Johannesson H, Engström P (2001) Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13. Plant Mol Biol 45:247–262
Hay A, Kaur H, Phillips A, Hedden P, Hake S, Tsiantis M (2002) The gibberellin pathway mediates KNOTTED1-type homeobox function in plants with different body plans. Curr Biol 12:1557–1565
Hay A, Craft J, Tsiantis M (2004) Plant hormones and homeoboxes: bridging the gap? Bioessays 26:395–404
Henriksson E, Olsson AS, Johannesson H, Johansson H, Hanson J, Engström P, Söderman E (2005) Homeodomain leucine zipper class genes in Arabidopsis. Expression patterns and phylogenetic relationships. Plant Physiol 139:509–518
Jasinski S, Piazza P, Craft J, Hay A, Woolley L, Rieu I, Phillips A, Hedden P, Tsiantis M (2005) KNOX action in Arabidopsis is mediated by coordinate regulation of cytokinin and gibberellin activities. Curr Biol 15:1560–1565
Johannesson N, Wang Y, Engström P (2001) DNA-binding and dimerization preferences of Arabidopsis homeodomain-leucine zipper transcription factors in vitro. Plant Mol Biol 45:63–73
Kerstetter RA, Laudencia-Chingcuanco D, Smith LG, Hake S (1997) Loss-of-function mutations in the maize homeobox gene, knotted1, are defective in shoot meristem maintenance. Development 124:3045–3054
Lee YH, Chun JY (1998) A new homeodomain-leucine zipper gene from Arabidopsis thaliana induced by water stress and abscisic acid treatment. Plant Mol Biol 37:377–384
McConnell JR, Barton MK (1998) Leaf polarity and meristem formation in Arabidopsis. Development 125:2935–2942
McConnell JR, Emery JF, Eshed Y, Bao N, Bowman J, Barton MK (2001) Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots. Nature 411:709–713
Morgan R (2006) Hox genes: a continuation of embryonic patterning? Trends Genet 22:67–69
Olsson AS, Engström P, Söderman E (2004) The homeobox genes ATHB12 and ATHB7 encode potential regulators of growth in response to water deficit in Arabidopsis. Plant Mol Biol 55:663–677
Olszewski N, Sun TP, Gubler F (2002) Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell 14:S61–S80
Prigge MJ, Wagner DR (2001) The Arabidopsis SERRATE gene encodes a zinc-finger protein required for normal shoot development. Plant Cell 13:1263–1279
Rueda EC, Dezar CA, Gonzales DH, Chan RL (2005) Hahb-10, a sunflower homeobox-leucine zipper gene, is regulated by light quality and quantity and promotes early flowering when expressed in Arabidopsis. Plant Cell Physiol 46:1954–1963
Sakamoto T, Kamiya N, Ueguchi-Tanaka M, Iwahori S, Matsuoka M (2001) KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem. Genes Dev 15:581–590
Schmitz G, Theres K (2005) Shoot and inflorescence branching. Curr Opin Plant Biol 5:506–511
Sessa G, Morelli G, Ruberti I (1993) The Athb-1 and -2 HD-Zip domains homodimerize forming complexes of different DNA binding specificities. EMBO J 12:3507–3517
Sessa G, Carabelli M, Ruberti I, Lucchetti S, Baima S, Morelli G (1994) Identification of distinct families of HD-Zip proteins in Arabidopsis thaliana. In: Pudigdomenech P, Coruzzi G (eds) Molecular-genetic analysis of plant development and metabolism. Springer, Heidelberg, pp 412–426
Söderman E, Hjellstrom M, Fahleson J, Engström P (1999) The HD-Zip gene ATHB6 in Arabidopsis is expressed in developing leaves, roots and carpels and up-regulated by water deficit conditions. Plant Mol Biol 40:1073–1083
Son O, Cho HY, Kim MR, Lee H, Lee MS, Song E, Park JH, Nam KH, Chun JY, Kim HJ, Hong SK, Chung YY, Hur CG, Cho HT, Cheon CI (2005) Induction of a homeodomain-leucine zipper gene by auxin is inhibited by cytokinin in Arabidopsis roots. Biochem Biophys Res Commun 326:203–209
Swain SM, Singh DP (2005) Tall tales from sly dwarves: novel functions of gibberellins in plant development. Trends Plant Sci 10:123–129
Tan QK-G, Irish VF (2006) The Arabidopsis zinc finger-homeodomain genes encode proteins with unique biochemical properties that are coordinately expressed during floral development. Plant Physiol 140:1095–1108
Vollbrecht E, Veit B, Sinha N, Hake S (1991) The developmental gene Knotted-1 is a member of a maize homeobox gene family. Nature 350:241–248
Xu YL, Li L, Wu K, Peeters AJM, Gage DA, Zeevaart JAD (1995) The GA5 locus of Arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. Proc Natl Acad Sci USA 92:6640–6644
Acknowledgments
This work was supported by a Korea Research Foundation Grant (KRF-2004-005-C00012) and the SRC/ERC program of MOST/KOSEF (Research Center for Women’s diseases; R11-2005-017). We are grateful to Dr M. Tsiantis for the se-2 (Col) mutant, and to Dr J. L. Bowman for the kan1 kan2 mutant.
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Communicated by J.R. Liu.
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Kim, YK., Son, O., Kim, MR. et al. ATHB23, an Arabidopsis class I homeodomain-leucine zipper gene, is expressed in the adaxial region of young leaves. Plant Cell Rep 26, 1179–1185 (2007). https://doi.org/10.1007/s00299-007-0340-9
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DOI: https://doi.org/10.1007/s00299-007-0340-9