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Micropropagation and germplasm conservation of Central Park Splendor Chinese elm (Ulmus parvifolia Jacq. ‘A/Ross Central Park’) trees

Plant Cell Reports volume 26, pages 1171–1177 (2007)Cite this article

Abstract

Micropropagation offers opportunities to propagate, preserve and ship tree germplasm. It also reduces the risk of moving pathogens and insects with the germplasm due to built-in pathogen detection capabilities of aseptic cultures. For the past few decades, our laboratory has been involved in a project to preserve and restore a large, cold hardy, and historically important Chinese elm (Ulmus parvifolia Jacq. ‘A/Ross Central Park’) tree. Here we present three simple and efficient systems for its micropropagation, germplasm conservation and distribution: (1) in vitro plant formation from meristematic nodules (MNs), (2) plantlet generation from axillary buds, and (3) in vitro rooting of micro-cuttings from 20-years-old hedged stock plants. Newly flushed nodal segments were used as explants. WPM with 0.5 mg/l BA was found to be the best medium for meristematic shoot development and WPM supplemented with 2.0 mg/l 4-CPPU and 0.5 mg/l TDZ was best for meristematic nodule formation. Rhizogenesis of regenerants and micro-cuttings was best achieved on WPM with 1.0 mg/l NAA and 2% sucrose. Rooted plants were readily acclimatized to the greenhouse ambient environment and continued to grow well under greenhouse conditions. The survival rate of acclimatized plantlets under ex vitro conditions was 100% after 4 weeks. Plants looked healthy with no visually detectable phenotypic variation based on observation of about 1,000 plants. Cycling of shoot explants and MNs through repetitive cultures was effective in scaling-up propagules.

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Abbreviations

BA:

Benzylaminopurine

MNs:

Meristematic nodules

PPFD:

Photosynthetic photon flux density

4-CPPU:

N-(2-chloro-4-pyridyl)-N′-phenylurea

TDZ:

1-Phenyl-3-(1,2,3-thidiazol-5-yl) urea (thidiazuron)

WPM:

Woody plant medium

NAA:

Naphthalene acetic acid

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Acknowledgments

This research was supported by the Arthur Ross Foundation, Inc.

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Authors and Affiliations

  1. School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, MI, 49931, USA

    R. C. Thakur & D. F. Karnosky

Authors
  1. R. C. Thakur
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  2. D. F. Karnosky
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Correspondence to R. C. Thakur.

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Communicated by S.A. Merkle.

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Thakur, R.C., Karnosky, D.F. Micropropagation and germplasm conservation of Central Park Splendor Chinese elm (Ulmus parvifolia Jacq. ‘A/Ross Central Park’) trees. Plant Cell Rep 26, 1171–1177 (2007). https://doi.org/10.1007/s00299-007-0334-7

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  • DOI: https://doi.org/10.1007/s00299-007-0334-7

Keywords

  • Chinese elm
  • Germplasm exchange
  • In vitro propagation
  • Meristematic nodules
  • Ulmus parvifolia

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