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Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

Plant Cell Reports volume 26, pages 823–835 (2007)Cite this article

Abstract

We isolated several senescence-associated genes (SAGs) from the petals of morning glory (Ipomoea nil) flowers, with the aim of furthering our understanding of programmed cell death. Samples were taken from the closed bud stage to advanced visible senescence. Actinomycin D, an inhibitor of transcription, if given prior to 4 h after opening, suppressed the onset of visible senescence, which occurred at about 9 h after flower opening. The isolated genes all showed upregulation. Two cell-wall related genes were upregulated early, one encoding an extensin and one a caffeoyl-CoA-3-O-methyltransferase, involved in lignin production. A pectinacetylesterase was upregulated after flower opening and might be involved in cell-wall degradation. Some identified genes showed high homology with published SAGs possibly involved in remobilisation processes: an alcohol dehydrogenase and three cysteine proteases. One transcript encoded a leucine-rich repeat receptor protein kinase, putatively involved in signal transduction. Another transcript encoded a 14-3-3 protein, also a protein kinase. Two genes have apparently not been associated previously with senescence: the first encoded a putative SEC14, which is required for Golgi vesicle transport, the second was a putative ataxin-2, which has been related to RNA metabolism. Induction of the latter has been shown to result in cell death in yeast, due to defects in actin filament formation. The possible roles of these genes in programmed cell death are discussed.

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Abbreviations

ActD:

Actinomycin D

ADH:

Alcohol dehydrogenase

DAPI:

4,6-Diamino-2-phenylindole

HR:

Hypersensitive response

LTP:

Lipid transfer protein

PCD:

Programmed cell death

RACE:

Rapid amplification of cDNA ends

SAG:

Senescence-associated gene

SCA2:

Spinocerebellar ataxia type 2

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Authors and Affiliations

  1. National Institute of Floricultural Science, 305-8519, Ibaraki, Japan

    Tetsuya Yamada & Kazuo Ichimura

  2. Tokyo University of Agriculture and Technology, 183-8509, Tokyo, Japan

    Tetsuya Yamada & Motoki Kanekatsu

  3. Wageningen University and Research Centre, PO Box 17, 6700, AA Wageningen, The Netherlands

    Wouter G. van Doorn

Authors
  1. Tetsuya Yamada
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  2. Kazuo Ichimura
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  3. Motoki Kanekatsu
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  4. Wouter G. van Doorn
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Correspondence to Tetsuya Yamada.

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Communicated by R. Schmidt

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Yamada, T., Ichimura, K., Kanekatsu, M. et al. Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers. Plant Cell Rep 26, 823–835 (2007). https://doi.org/10.1007/s00299-006-0285-4

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  • DOI: https://doi.org/10.1007/s00299-006-0285-4

Keywords

  • Actinomycin D
  • DNA degradation
  • Nuclear fragmentation
  • Petal senescence
  • Programmed cell death
  • Senescence-associated genes