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Micropropagation of photinia employing rhizobacteria to promote root development

  • Cell Biology and Morphogenesis
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Abstract

An alternative protocol was developed for in vitro propagation of photinia (Photinia × fraseri Dress), an ornamental shrub, using the plant growth-promoting rhizobacteria (PGPR) Azospirillum brasilense and Azotobacter chroococcum during rhizogenesis. Shoot tips from four-year-old mature plants, cut in spring and summer, were used as initial explants. They were cultured on Murashige–Skoog (MS) medium with Gamborg’s vitamins, N6-benzyladenine (BA: 11.1 μM) and gibberellic acid (GA3: 1.3 μM), obtaining 63% of established explants. The highest shoot length (22.9 mm) and multiplication rate (4.3) was achieved by cultivating for four weeks in the same basal medium supplemented with 4.4 μM BA. Both auxin induction and bacterial inoculation were used for rooting. Elongated shoots were treated with two concentrations of indole-3-butyric acid (IBA: 4.9 or 49.2 μM) during 6 days for auxin induction. Then, the shoots were transferred to an auxin-free medium and inoculated with A. brasilense Cd, Sp7 or A. chroococcum (local strain). Bacterial inoculation induced earlier rooting of photinia shoots. A. brasilense Cd with 49.2 μM IBA pulse showed a significant increase (P ≤ 0.05) in root fresh and dry weight (105%, 137%), root surface area (65%) and shoot fresh and dry weight (32%, 62%). A. brasilense Sp7 enhanced the root fresh weight (34%) and root surface area (41%) while no significant differences with A. chroococcum inoculation were detected. The PGPR inoculated micro-cuttings in combination with auxin induction pulses may play a useful role in root organogenesis of micropropagated plants.

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Abbreviations

ATCC:

American Type Culture Collection

BA:

N6-benzyladenine

c.f.u.:

Colony forming unit

GA3 :

Gibberellic acid A3

IBA:

Indole-3-butyric acid

PGPR:

Plant growth-promoting rhizobacteria

References

  • Barka EA, Belarbi A, Hachet C, Nowak J, Audran JC (2000) Enhancement of in vitro growth and resistance to gray mould of Vitis vinifera co-cultured with plant growth-promoting rhizobacteria. FEMS Microbiol Lett 186:91–95. DOI 10.1111/j.1574-6968.2000.tb09087.x

    Article  PubMed  CAS  Google Scholar 

  • Beeson RC (2000) Putting the speed back in quick-dip auxin application. SNA Res Conf 45:298–302

    Google Scholar 

  • Brickell C (1996) Enciclopedia de Plantas y Flores. Royal horticultural society, Grijalbo Mondadori, Verona, Italy, pp 566

    Google Scholar 

  • Brown ME, Burlingham SK, Jackson RM (1962) Studies on Azotobacter species in soil. 1: Comparison of media and techniques for counting Azotobacter in soil. Plant Soil 17:309–313. DOI 10.1007/BF01377670

    Article  Google Scholar 

  • Burns JA, Schwarz OJ (1996) Bacterial stimulation of adventitious rooting on in vitro cultured slash pine (Pinus elliottii Engelm.) seedling explants. Plant Cell Rep 15:405–408. DOI 10.1007/BF00232064

    Article  CAS  Google Scholar 

  • Capellades-Queralt M, Beruto AM, Vanderschaeghe A, Debergh PC (1993) Ornamentals. In: Debergh PC, Zimmerman RH (eds) Micropropagation, technology and application. Kluwer, The Netherlands, pp 215–229

    Google Scholar 

  • Carletti SM, Llorente B, Rodríguez Cáceres E, Tandecarz J (1998) Jojoba inoculation with Azospirillum brasilense stimulates in vitro root formation. Plant Tissue Cult Biotech 4:165–174

    Google Scholar 

  • Carley HE, Watson RD (1966) A new gravimetric method for estimating root-surface areas. Soil Sci 102:289–291

    Article  Google Scholar 

  • Frommel M, Nowak J, Lazarovits G (1991) Growth enhancement and developmental modifications of in vitro grown potato (Solanum tuberosum spp. tuberosum) as affected by a non fluorescent Pseudomonas sp. Plant Physiol 96:928–936

    Article  PubMed  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158. DOI 10.1016/0014-4827(68)90403-5

    Article  PubMed  CAS  Google Scholar 

  • Kane ME, Sheehan TJ, Philman NL (1987) A micropropagation protocol using fraser photinia for mutation induction and new cultivar selection. Proc Fla State Hort Soc 100:334–337

    Google Scholar 

  • Leifert C, Pryce S, Lumsden PJ, Waites WM (1992) Effects of medium acidity on growth and rooting of different plant species growing in vitro. Plant Cell Tiss Org Cult 30:171–179. DOI 10.1007/BF00040019

    Article  Google Scholar 

  • Llorente BE, Apóstolo NM (1998) Effect of different growth regulators and genotype on in vitro propagation of jojoba. New Zealand J Crop Hort Sci 26:55–62

    CAS  Google Scholar 

  • Merkle SA, Dean JF (2000) Forest tree biotechnology. Curr Opin Biotechnol 11:298–302. DOI: 10.1016/S0958-1669(00)00099-9

    Article  PubMed  CAS  Google Scholar 

  • Mirza MS, Ahmad W, Latif F, Haurat J, Bally R, Normand P, Mallik KA (2001) Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micropropagated sugarcane in vitro. Plant Soil 237:47–54. DOI 10.1023/A:1013388619231

    Article  CAS  Google Scholar 

  • Modgil M, Sharma DR, Bhardwaj SV (1999) Micropropagation of apple cv. Tydeman’s Early Worcester Sci Hort 81:179–188. DOI 10.1016/S0304-4238(98)00259-3

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nowak J, Shulaev J (2003) Priming for transplant stress resistance in in vitro propagation. In Vitro Cell Dev Biol Plant 39:107–124. DOI 10.1079/IVP2002403

    Google Scholar 

  • Nowak J (1998) Benefits of in vitro “biotization” of plant tissue cultures with microbial inoculants. In Vitro Cell Dev Biol Plant 34:122–130

    Google Scholar 

  • Okon Y, Albrecht SL, Burris RH (1977) Methods for growing Spirillum lipoferum and for counting it in pure culture and in association with plants. Appl Environ Microbiol 33:85–88

    PubMed  Google Scholar 

  • Okon Y, Vanderleyden J (1997) Root-associated Azospirillum species can stimulate plants. ASM News 63:366–370

    Google Scholar 

  • Ramírez-Malagón R, Borodanenko A, Barrera-Guerra J, Ochoa-Alejo N (1997) Micropropagation for fraser photinia (Photinia × fraseri). Plant Cell Tiss Org Cult 48:219–222. DOI 10.1023/A:1005898106134

    Article  Google Scholar 

  • Shetty K, Carpenter TL, Curtis OF, Potter TL (1996) Reduction of hyperhydricity in tissue cultures of oregano (Origanum vulgare) by extracellular polysaccharide isolated from Pseudomonas spp. Plant Sci 120:175–183. DOI 10.1016/S0168-9452(96)04482-2

    Article  CAS  Google Scholar 

  • Shibli RA, Ajlouni MM, Jaradat A, Aljanabi S, Sharnawi M (1997) Micropropagation in wild pear (Pyrus syrica). Sci Hort 68:237—242. DOI 10.1016/S0304-4238(96)00972-7

    Article  CAS  Google Scholar 

  • Vestberg M, Kukkonen S, Saari K, Parikka P, Huttunen J, Tainio L, Devos N, Weekers F, Kevers C, Thonart P, Lemoine MC, Cordier C, Alabouvette C, Gianinazzi S (2004) Microbial inoculation for improving the growth and health of micropropagated strawberry. Appl Soil Ecol 27:243–258. DOI 10.1016/j.apsoil.2004.05.006

    Article  Google Scholar 

  • von Aderkas P, Bonga JM (2000) Influencing micropropagation and somatic embryogenesis in mature trees by manipulation of phase change, stress and culture environment. Tree Physiol 20:921–928

    PubMed  CAS  Google Scholar 

  • Zimmerman RH, Jones JB (1991) Commercial micropropagation in North America. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer, The Netherlands, pp 173–179

    Google Scholar 

Download references

Acknowledgements

The authors thank Lic. Susana Filippini from Statistical Division, National University of Luján, for providing statistical advice. This research was supported by a grant from the Department of Basic Sciences, National University of Luján, Argentina.

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Basic Sciences, National University of Luján, Rutas 5 y 7, CC 221, B6700, Luján, Buenos Aires, Argentina

    Ezequiel E. Larraburu, Susana M. Carletti, Enrique A. Rodríguez Cáceres & Berta E. Llorente

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  1. Ezequiel E. Larraburu
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  2. Susana M. Carletti
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  3. Enrique A. Rodríguez Cáceres
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  4. Berta E. Llorente
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Correspondence to Berta E. Llorente.

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Communicated by H. S. Judelson

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Larraburu, E.E., Carletti, S.M., Rodríguez Cáceres, E.A. et al. Micropropagation of photinia employing rhizobacteria to promote root development. Plant Cell Rep 26, 711–717 (2007). https://doi.org/10.1007/s00299-006-0279-2

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