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compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice (Oryza sativa L.)

Plant Cell Reports volume 26, pages 421–427 (2007)Cite this article

Abstract

The shoot apical meristem (SAM) produces lateral organs in a regular spacing (phyllotaxy) and at a regular interval (phyllochron) during the vegetative phase. In a Dissociation (Ds) insertion rice population, we identified a mutant, compact shoot and leafy head 1 (csl1), which produced massive number of leaves (∼70) during the vegetative phase. In csl1, the transition from the vegetative to the reproductive phase was delayed by about 2 months under long-day conditions. With a reduced leaf size and severe dwarfism, csl1 failed to produce a normal panicle after the transition to reproductive growth. Instead, it produced a leafy panicle, in which all primary rachis-branches were converted to vegetative shoots. Phenotypically csl1 resembled pla mutants in short plastochron but was more severe in the conversion of the reproductive organs to vegetative organs. In addition, neither the expression nor the coding region of PLA1 or PLA2 was affected in csl1. csl1 is most likely a dominant mutation because no mutant segregant was observed in progeny of 67 siblings of the csl1 mutant. CSL1 may represent a novel gene, which functions downstream of PLA1 and/or PLA2, or alternatively functions in a separate pathway, involved in the regulation of leaf initiation and developmental transition via plant hormones or other mobile signals.

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Abbreviations

AM:

axillary meristem

Ds :

Dissociation

Dt:

double-transformation

GFP:

green fluorescent protein

iAc :

immobile Activator

IM:

inflorescence meristem

SAM:

shoot apical meristem

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Acknowledgements

The authors thank Kerrie Ramm, Ramani Shivakkumar and Shamsul Hoque for generating the transgenic line, and Celia Miller for SEM observation. We also thank Dr. Ramesh Bhat for critical reading. This work was partially supported by GrainGene1 (Australia), and NSW Agricultural Genomics Centre.

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Affiliations

  1. CSIRO Plant Industry, Australian Capital Territory, 2601, Canberra, Australia

    Qian-Hao Zhu, Elizabeth S. Dennis & Narayana M. Upadhyaya

  2. New South Wales Agricultural Genomics Centre, Wagga Wagga, New South Wales, 2678, Australia

    Qian-Hao Zhu, Elizabeth S. Dennis & Narayana M. Upadhyaya

Authors
  1. Qian-Hao Zhu
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  2. Elizabeth S. Dennis
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  3. Narayana M. Upadhyaya
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Corresponding author

Correspondence to Narayana M. Upadhyaya.

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Communicated by P. Lakshmanan

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Zhu, QH., Dennis, E.S. & Upadhyaya, N.M. compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice (Oryza sativa L.). Plant Cell Rep 26, 421–427 (2007). https://doi.org/10.1007/s00299-006-0259-6

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  • DOI: https://doi.org/10.1007/s00299-006-0259-6

Keywords

  • compact shoot and leafy head 1
  • Developmental transition
  • Leaf initiation
  • Phyllochron
  • Rice