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In vitro regeneration of wild pear (Pyrus pyraster Burgsd) clones tolerant to Fe-chlorosis and somaclonal variation analysis by RAPD markers

  • Genetics and Genomics
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Abstract

An in vitro adventitious regeneration system under selective pressure was established in Pyrus pyraster Burgsd to obtain somaclones with higher adaptability to calcareous soils. P. pyraster is important species, both for its relative closeness to cultivated pear and for reforestation of marginal farmland and for the production of timber. Shoot regeneration was induced from leaves and vegetative apices of in vitro-grown shoots on a modified LP medium supplemented with naphtaleneacetic acid (1.07 μM) and benziladenine (BA, 8.9 μM). After 30 days, explants were transferred to an expression medium consisting of the same basal medium with only BA present. Selective treatments utilized MS medium with Fe-EDTA replaced by equimolar amount of FeSO4 with either KHCO3 or NaHCO3. Through the selection process 11 putatively tolerant lines were obtained from vegetative shoot apices. RAPD analysis was performed on these lines to allow comparison to the mother clone. A total of seven 10-mer primers were used to amplify all the genotypes and 74 scorable fragments were produced. These were analysed using the Dice similarity index, showing genetic variability among the 11 regenerated clones and between them and the mother clone.

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Abbreviations

BA:

Benzyladenine

EX:

Expression phase

IND:

Induction phase

LE:

Leaf explant

LP:

Quoirin et al.

MULT:

Multiplication

SAE:

Shoot apex explants

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Authors and Affiliations

  1. CRA–Istituto Sperimentale per la Frutticoltura, Via di Fioranello 52, 00134, Rome, Italy

    Maria Antonietta Palombi, Beatrice Lombardo & Emilia Caboni

Authors
  1. Maria Antonietta Palombi
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  2. Beatrice Lombardo
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  3. Emilia Caboni
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Correspondence to Maria Antonietta Palombi.

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Communicated by P. Puigdoménech

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Palombi, M.A., Lombardo, B. & Caboni, E. In vitro regeneration of wild pear (Pyrus pyraster Burgsd) clones tolerant to Fe-chlorosis and somaclonal variation analysis by RAPD markers. Plant Cell Rep 26, 489–496 (2007). https://doi.org/10.1007/s00299-006-0256-9

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  • DOI: https://doi.org/10.1007/s00299-006-0256-9

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