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High frequency early in vitro flowering of Dendrobium Madame Thong-In (Orchidaceae)

  • Cell Biology and Morphogenesis
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Abstract

We have successfully developed a method to induce early in vitro flowering of the self-pollinated seedlings of a tropical orchid hybrid, Dendrobium Madame Thong-In. Transition of vegetative shoot apical meristem to inflorescence meristem was observed when young protocorms were cultured in modified KC liquid medium. In contrast, protocorms cultured on Gelrite-solidified medium only produced axillary shoots and roots. CW was required to trigger the transitional shoot apical meristem and BA enhanced inflorescence stalk initiation and flower bud formation. However, normal flower development was deformed in liquid medium but developed fully upon transferring to two-layered (liquid over Gelrite-solidified) medium. Under optimal condition, in vitro flowering was observed about 5 months after seed sowing. Segregation of flower colours was observed in these seedlings and seedpods formed upon artificial pollination of the in vitro flowers.

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Abbreviations

AC:

Activated charcoal

BA:

6-Benzyladenine

CW:

Coconut water

KR:

Gelrite-solidified medium

KRA:

Gelrite-solidified medium with AC

K0:

Basal liquid medium

K1:

Basal liquid medium with 4.4 μM BA

K5:

Basal liquid medium with 22.2 μM BA

K5RA:

Gelrite-solidified medium with 22.2 μM BA and AC

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Acknowledgements

G. E. Sim was supported by a research scholarship from the National University of Singapore (NUS). C. S. Loh thanks the NUS for a research grant. We thank Professor Prakash Kumar for critical reading of this manuscript.

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Author notes

  1. Guek Eng Sim

    Present address: Plant Biotechnology and Agrotechnology Section, School of Chemical and Life Sciences, Singapore Polytechnic, 500 Dover Road, 139651, Singapore, Singapore

Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, Science Drive 4, 117543, Singapore, Singapore

    Guek Eng Sim, Chiang Shiong Loh & Chong Jin Goh

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  1. Guek Eng Sim
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  2. Chiang Shiong Loh
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  3. Chong Jin Goh
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Correspondence to Chiang Shiong Loh.

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Communicated by K. K. Kamo

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Sim, G.E., Loh, C.S. & Goh, C.J. High frequency early in vitro flowering of Dendrobium Madame Thong-In (Orchidaceae). Plant Cell Rep 26, 383–393 (2007). https://doi.org/10.1007/s00299-006-0242-2

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  • DOI: https://doi.org/10.1007/s00299-006-0242-2

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