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Thidiazuron-induced regeneration of Echinacea purpurea L.: Micropropagation in solid and liquid culture systems

  • Cell Biology and Morphogenesis
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Abstract

The goals of this study were to investigate thidiazuron (TDZ)-induced morphogenesis of Echinacea purpurea L. and to assess the possibility of developing a liquid-based protocol for rapid micropropagation. Callus development and root organogenesis were observed on leaf explants cultured on media containing 2,4-dicholorophenoxyacetic acid or dicamba, but no plantlets were regenerated. Addition of TDZ to the culture medium as the sole growth regulator resulted in the production of regenerable callus cultures. The highest rate of regeneration was observed for explants cultured on medium with TDZ at 2.5 μM or higher. Tissue derived from 1.0 μM TDZ treatments was used to initiate liquid cultures. All liquid treatments produced a similar number of regenerants but significantly more healthy plants were obtained from cultures grown in the presence of 0.1 and 1.0 μM TDZ. This TDZ-based micropropagation system is the first liquid, large-scale propagation protocol developed for the mass production of E. purpurea plants.

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Abbreviations

2,4-D:

2,4-dicholorophenoxyacetic

DC:

dicamba

TDZ:

thidiazuron

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Acknowledgments

The financial contributions from the Natural Science and Engineering Research Council of Canada and Science-Based Medicinal Plants Inc., Dundas, Ontario are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Maxwell P. A. Jones, Zhijun Yi & Praveen K. Saxena

  2. Chemistry, Earth and Environmental Sciences, University of British Columbia Okanagan, Kelowna, BC, V1V 1V7, Canada

    Susan J. Murch

Authors
  1. Maxwell P. A. Jones
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  2. Zhijun Yi
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  3. Susan J. Murch
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  4. Praveen K. Saxena
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Corresponding author

Correspondence to Praveen K. Saxena.

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Communicated by P. Ozias-Akins

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Jones, M.P.A., Yi, Z., Murch, S.J. et al. Thidiazuron-induced regeneration of Echinacea purpurea L.: Micropropagation in solid and liquid culture systems. Plant Cell Rep 26, 13–19 (2007). https://doi.org/10.1007/s00299-006-0209-3

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  • DOI: https://doi.org/10.1007/s00299-006-0209-3

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