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Leaf senescence is delayed in tobacco plants expressing the maize knotted1 gene under the control of a wound-inducible promoter

  • Physiology and Biochemistry
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Abstract

To extend the shelf life of freshly harvested vegetables and cut flowers, a maize homeobox gene Knotted1 (kn1) was placed under the control of a wound-inducible promoter win3.12 from hybrid poplar (Populus trichocarpa × P. deltoides) and introduced into tobacco plants (Nicotiana tabacum cv. Xanthi). Transgenic win3.12::kn1 plants were morphologically normal. A leaf-detachment assay demonstrated that senescence in win3.12::kn1 leaves could be delayed by at least 2 weeks compared with wild type leaves. Furthermore, all leaves of win3.12::kn1 shoots remained green and healthy 3 weeks after excision and incubation in water, while older leaves of control shoots senesced under the same conditions. Additionally, a number of adventitious roots produced at the cut ends of wild type shoots after a 3-week incubation, but much a less number of adventitious roots appeared in win3.12::kn1 shoots. The delay in senescence was also confirmed by a higher total chlorophyll (a + b) content in win3.12::kn1 leaves relative to that of the control plants. RT-PCR analysis showed that the kn1 transcript was detected in win3.12::kn1 leaves with wounding treatment, but otherwise was not observed in leaves of wild type and unwounded transgenic plants. The results presented here indicate that expression of kn1 gene driven by the wound-inducible promoter win3.12 is potentially useful to delay senescence of vegetable crops and commercial horticulture after harvest.

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Abbreviations

GUS :

Glucuronidase

CaMV 35S :

Cauliflower mosaic virus 35S

kn1 :

Knotted1 gene

BA :

Benzylaminopurine

NAA :

Naphthaleneacetic acid

nptII :

Neomycin phosphotransferase

MS Medium :

Musashige & Skoog medium

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Acknowledgements

We wish to thank Dr. Yongqing Chen for critically reading the manuscript and excellent scientific discussions. This work was supported by a grant from the National Natural Science Foundation of China (#30300225).

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Authors and Affiliations

  1. Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China

    Keming Luo, Wei Deng, Yuehua Xiao, Xuelian Zheng & Yan Pei

  2. Department of Plant Science, University of Connecticut, 1376 Storrs Road U-4067, Storrs, CT, 06269-4067, USA

    Yi Li

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  1. Keming Luo
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  2. Wei Deng
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  3. Yuehua Xiao
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  4. Xuelian Zheng
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Correspondence to Yan Pei.

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Communicated by P. Lakshmanan

The first two authors contributed equally to this work.

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Luo, K., Deng, W., Xiao, Y. et al. Leaf senescence is delayed in tobacco plants expressing the maize knotted1 gene under the control of a wound-inducible promoter. Plant Cell Rep 25, 1246–1254 (2006). https://doi.org/10.1007/s00299-006-0178-6

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  • DOI: https://doi.org/10.1007/s00299-006-0178-6

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