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Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes

Plant Cell Reports volume 25, pages 1059–1066 (2006)Cite this article

Abstract

We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica. Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments. However, hormonal treatment was required to regenerate shoots in root explants of wild type control plants. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes, which include, short internodes, small and wrinkled leaves, more branches and numerous plagiotropic roots. Plants regenerated from transformed roots showed increased biomass accumulation (350–510% in the roots and 200–320% in the whole plants) and augmented tylophorine content (20–60%) in the shoots, resulting in a 160–280% increase in tylophorine production in different clones grown in vitro.

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Abbreviations

BA:

6-Benzyl adenine

BM:

Basal medium

HPLC:

High performance liquid chromatography

Ri:

Root-inducing

T-DNA:

Transferred DNA

TL-DNA:

Left-handed T-DNA

WT:

Wild type

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Acknowledgements

We thank the Programme Coordinator, Centre of Advanced Study, Department of Botany, University of Calcutta for the facilities provided, Dr. F. Abe, Fukuoka University, Japan for the standard tylophorine samples, Dr. A. Kollman, INRA, Versailles, France for his support in tylophorine analysis and Mr. N. Roy for photography. This work was funded by the Indo-French Centre for the Promotion of Advanced Research (Centre Franco-Indien pour la Promotion de la Recherche Avancée) and the Department of Science and Technology, Government of India.

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Affiliations

  1. Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Calcutta, 700019, India

    Kuntal Narayan Chaudhuri & Sumita Jha

  2. Department of Botany, RKMVC College, Rahara, 24 Parganas (North), West Bengal, India

    Biswajit Ghosh

  3. Laboratoire de Biologie de la Rhizosphère (Phytopharmacie), Institut National de la Recherche Agronomique, 78026, Versailles cédex, France

    David Tepfer

Authors
  1. Kuntal Narayan Chaudhuri
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  2. Biswajit Ghosh
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  3. David Tepfer
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  4. Sumita Jha
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Corresponding author

Correspondence to Sumita Jha.

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Communicated by P. Lakshmanan

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Chaudhuri, K.N., Ghosh, B., Tepfer, D. et al. Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes . Plant Cell Rep 25, 1059–1066 (2006). https://doi.org/10.1007/s00299-006-0164-z

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Keywords

  • Agrobacterium rhizogenes
  • Medicinal plants
  • Plant regeneration
  • Tylophora indica
  • Tylophorine