Plant Cell Reports

, Volume 25, Issue 9, pp 993–996 | Cite as

Thymol derivatives from hairy roots of Arnica montana

  • I. Weremczuk-Jeżyna
  • W. Kisiel
  • H. WysokińskaEmail author
Physiology and Biochemistry


Five known thymol derivatives were isolated from roots of Arnica montana transformed with Agrobacterium rhizogenes LBA 9402. The compounds were characterized by spectral methods. The pattern of thymol derivatives in light-grown hairy roots was slightly different from that in dark-grown ones. This is the first report on the presence of thymol derivatives in hairy roots of the plant.


Arnica montana Asteraceae Hairy root culture Thymol derivatives 



This investigation was supported by a research grant 503-312-1 from Medical University of Łódź.


  1. Bohlmann F, Zdero C (1972) Neue Thymol-Derivate aus Arnica amplexicaulis. Tetrahedron Lett 28:2827–2828CrossRefGoogle Scholar
  2. Bohlmann F, Niedballa U, Schülz J (1969) Über einige Thymolderivative aus Gaillardia- und Helenium-Arten. Chem Ber 102:864–871CrossRefGoogle Scholar
  3. Bohlmann F, Jakupovic J, Lonitz M (1977) Uber Inhaltsstoffe der Eupatorium—Gruppe. Chem Ber 110:301–314CrossRefGoogle Scholar
  4. Bohlmann F, Jakupovic J, King RM, Robinson H (1982) New labdane derivatives from Madia sativa. Phytochemistry 21:1103–1107CrossRefGoogle Scholar
  5. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158CrossRefPubMedGoogle Scholar
  6. Gonzalez A, Barrera JB, Rosas FE, Hernandez ACY, Espineira J, Joseph-Nathan P (1986) Thymol derivatives from Schizogyne glaberrima. Phytochemistry 25:2889–2891CrossRefGoogle Scholar
  7. Herz W, Sosa E (1988) Sesquiterpene lactones and other constituents of Arnica acaulis. Phytochemistry 27:155–159CrossRefGoogle Scholar
  8. Hosokawa K, Matsuki R, Oikawa Y, Yamamura S (1997) Genetic transformation of gentian using wild-type Agrobacterium rhizogenes. Plant Cell Tissue Organ Cult 51:137–140CrossRefGoogle Scholar
  9. Maldonado E, Marquez CL, Ortega A (1992) A thymol derivative from Calea nelsonii. Phytochemistry 31:2527–2528CrossRefGoogle Scholar
  10. Metwally MA, Dawidar AM (1985) A thymol derivative from Inula crithmoides. Phytochemistry 24:1377–1378CrossRefGoogle Scholar
  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  12. Passreiter CM, Matthiesen U, Willuhn G (1998) 10-Acetoxy-9-chloro-8,9-dehydrothymol and further thymol derivatives from Arnica sachalinensis. Phytochemistry 49:777–781CrossRefGoogle Scholar
  13. Petit A, David C, Dahl JG, Ellis P, Guyon P, Casse-Delbard F, Tempe J (1983) Further extension of opine concept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190:2004–2014CrossRefGoogle Scholar
  14. Rossetti V, Lombard A, Sancin P, Buffa M, Di Stefano R (1984) Characterization of Arnica montana L. dried roots. Int J Crude Drug Res 22:53–60Google Scholar
  15. Su BN, Takaishi Y, Yabuuchi T, Kusumi T, Tori M, Takaoka S, Honda G, Ito M, Takeda Y, Kodzhimatov OK, Ashurmetov O (2001) Sesquiterpenes and monoterpenes from bark of Inula macrophylla. J Nat Prod 64:466–471CrossRefPubMedGoogle Scholar
  16. Tori M, Ohara Y, Nakashima K, Sono M (2001) Thymol derivatives from Eupatorium fortunei. J Nat Prod 64:1048–1051CrossRefPubMedGoogle Scholar
  17. Vervliet G, Holsters M, Teuchy H, Montagi M, Schell J (1975) Characterization of different plaque-forming and defective temperate pharges in Agrobacterium strains. J Gen Virol 26:33–48PubMedCrossRefGoogle Scholar
  18. Wagner S, Suter A, Merfort I (2004) Skin penetration studies of Arnica preparations and of their sesquiterpene lactones. Planta Med 70:897–903CrossRefPubMedGoogle Scholar
  19. Weremczuk-Jeżyna I, Wysokińska H (2004) Kultury korzeni transformowanych Arnica montana—izolacja metabolitów wtórnych. Biotechnologia 2:54–60Google Scholar
  20. Willuhn G (1972) Untersuchungen über die Inhaltsstoffe von Arnica—Arten. VII. Die Zusammensetzung des ätherischen Öles aus den unterirdischen Organen und Blütenkörbchen verschiedener Arnica—Arten. Planta Med 22:1–33PubMedCrossRefGoogle Scholar
  21. Willuhn G (1998) Arnica flowers: pharmacology, toxicology and analysis of the sesquiterpene lactones—their main active substances. ACS Symp Ser 691 Am Chem Soc 118–132Google Scholar
  22. Zee OP, Kim DK, Lee KR (1998) Thymol derivatives from Carpesium divaricatum. Arch Pharm Res 21:618–620PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • I. Weremczuk-Jeżyna
    • 1
  • W. Kisiel
    • 2
  • H. Wysokińska
    • 1
    Email author
  1. 1.Department of Biology and Pharmaceutical BotanyMedical University of ŁódźŁódźPoland
  2. 2.Department of PhytochemistryInstitute of Pharmacology, Polish Academy of SciencesKrakówPoland

Personalised recommendations