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Mannose selection system used for cucumber transformation

  • Genetic Transformation and Hybridization
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Abstract

The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10 g/l mannose and 10 g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation.

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Acknowledgements

The authors would like to thank Syngenta Corp. for providing pmi gene. This project was supported by a grant from Hubei Provincial Department of Education (2004Q001), a grant from the Office of Science and Technology of Yichang (A04401-12), and a grant from China Three Gorges University (KJB0302).

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Authors and Affiliations

  1. Biotechnology Research Center, China Three Gorges University, Yichang, 443002, P.R. China

    Zhengquan He, ZhenZhen Duan, Wei Liang, Faju Chen, Wei Yao, Hongwei Liang, Chaoyin Yue, Fan Chen & Jianwu Dai

  2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, P.R. China

    Fan Chen & Jianwu Dai

  3. National Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou, 310006, P.R. China

    Zongxiu Sun

Authors
  1. Zhengquan He
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  2. ZhenZhen Duan
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  3. Wei Liang
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  4. Faju Chen
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  5. Wei Yao
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  6. Hongwei Liang
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  7. Chaoyin Yue
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  9. Fan Chen
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  10. Jianwu Dai
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Corresponding author

Correspondence to Jianwu Dai.

Additional information

Communicated by D. Dudits

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He, Z., Duan, Z., Liang, W. et al. Mannose selection system used for cucumber transformation. Plant Cell Rep 25, 953–958 (2006). https://doi.org/10.1007/s00299-006-0156-z

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  • DOI: https://doi.org/10.1007/s00299-006-0156-z

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