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In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.)

  • Cell Biology and Morphogenesis
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Abstract

Regeneration of adventitious shoots from leaves and, for the first time, from internode sections were compared and optimized for five economically important sweet cherry cultivars, i.e. ‘Schneiders’, ‘Sweetheart’, ‘Starking Hardy Giant’, ‘Kordia’ and ‘Regina’ (Prunus avium L.). The influence of basal media, carbon source, combination and dosage of phytohormones, ethylene inhibitor such as silver thiosulfate and a 16 h:8 h light:dark photoperiod versus complete darkness were evaluated. Both, DKW/WPM (1:1) and Quoirin/Lepoivre (QL) basal media stimulated organogenesis more than QL/WPM (1:1), Chee and Pool (CP), Murashige Skoog (MS), Driver and Kuniyuki (DKW) or woody plant (WPM) media did. An induction phase in darkness resulted in lower or zero regeneration rates. The best regeneration efficiencies were generally obtained with thidiazuron in combination with indole-3-butyric-acid. The addition of silver thiosulfate resulted in a similar or reduced regeneration efficiency. Significant genotypic variability in adventitious bud formation was evident for both explant sources, leaf and internode section. Adventitious shoots were obtained from 11% of leaf explants and 50% of internode sections indicating that shoot regeneration from internodes was significantly more efficient than from leaves.

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Abbreviations

BAP:

N6-benzylamino-purine;

CP:

Chee and Pool medium;

DKW:

Driver and Kuniyuki medium;

Fe-EDDHA:

ethylene-diamine-ferric;

IAA:

indole-3-acetic-acid;

IBA:

indole-3-butyric-acid;

MS:

Murashige Skoog medium;

NAA:

α-naphtaleneacetic-acid;

QL:

Quoirin/Lepoivre medium;

TDZ:

1-Phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron);

WPM:

Woody plant medium

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Acknowledgements

This research was funded by a grant of the Federal Ministry of Education and Research (BMBF 0312217). We thank Ute Poh for excellent assistance in subculturing the sweet cherry shoot cultures.

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  1. Laboratory for Biotechnological Crop Protection, Department of Phytopathology, Agricultural Service Center Palatinate, 67435, Neustadt an der Weinstraße, Germany

    Andrea Matt & Johannes A. Jehle

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  1. Andrea Matt
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  2. Johannes A. Jehle
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Correspondence to Johannes A. Jehle.

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Communicated by H. Lörz

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Matt, A., Jehle, J.A. In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.). Plant Cell Rep 24, 468–476 (2005). https://doi.org/10.1007/s00299-005-0964-6

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  • DOI: https://doi.org/10.1007/s00299-005-0964-6

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