Skip to main content

An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants

Plant Cell Reports volume 23, pages 775–779 (2005)Cite this article

Abstract

The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves were explanted onto Woody Plant Medium and maintained initially in the dark. The plant growth regulator thidiazuron (TDZ) was effective in stimulating shoot regeneration from leaf explants of Zhanhua winter jujube. The highest efficiency of shoot formation was observed with a 20-day culture in the dark on WPM containing 4.54 μM TDZ and 2.85 μM indoleacetic acid (IAA). The regenerated shoots were transferred to MS medium containing 0.89 μM benzyladenine and 5.77 μM gibberellic acid for growth. When the shoots were about 2 cm in height, they were transferred to Nitsch medium supplemented with 1.14 μM IAA and 2.46 μM indolebutyric acid to induce rooting. This system of adventitious shoot production from leaf explants of adult plants could be useful for the genetic engineering and polyploidization of winter jujube.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1

Abbreviations

AS :

Average number of shoots per explant

BA :

Benzyladenine

GA 3 :

Gibberellic acid

IAA :

Indoleacetic acid

IBA :

Indolebutyric acid

LPS :

Percentage of leaf explants producing shoots

NAA :

α-Naphthaleneacetic acid

TDZ :

Thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-ylurea)

References

  • Arzate-Fernandez A, Nakazaki T, Yamagata H, Tanisaka T (1997) Production of double-haploid plants from Lilium longiflorum Thunb. anther culture. Plant Sci 123:179–187

    Article  Google Scholar 

  • Carneiro LA, Araujo RFG, Brito GJM, Fonseca MHPB, Costa A, Crocomo OJ, Mansur E (1999) In vitro regeneration from leaf explants of Neoregelia cruenta (R. Graham) L.B. Smith, an endemic bromeliad from Eastern Brazil. Plant Cell Tissue Organ Cult 55:79–83

    Article  Google Scholar 

  • Chen ZL, Yan ZL, Xue H, Feng XD, Chen CL, Cao JY (2002) In vitro culture of leaves and plantlets regeneration of Zizyphus jujuba var. Zhanhua-Dongzao. Plant Physiol Commun 38:584

    Google Scholar 

  • Cutler AJ, Krochko JE (1999) Formation and breakdown of ABA. Trends Plant Sci 4:472–478

    Article  PubMed  Google Scholar 

  • Fasolo F, Zimmerman RH, Fordham I (1989) Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant Cell Tissue Organ Cult 16:75–87

    CAS  Google Scholar 

  • Hare PD, van Staden J (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol 35:1121–1125

    Google Scholar 

  • Hossain SN, Munshi MK, Islam MR, Hakim L, Hossain M (2003) In vitro propagation of plum (Zyziphus jujuba Lam). Plant Tissue Cult 13:81–84

    Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119

    CAS  Google Scholar 

  • Leblay C, Chevreau E, Raboin LM (1991) Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.). Plant Cell Tissue Organ Cult 25:99–105

    Google Scholar 

  • Li HW, Feng SQ, Zhao YM (1999) Primary study on the storage of Chinese jujube dongzao. J Shanxi Agric Sci 27:65–67

    Google Scholar 

  • Lin HS, De Jeu MJ, Jacobsen E (1998) Formation of shoots from leaf axils of Alstroemeria: the effect of the position on the stem. Plant Cell Tissue Organ Cult 52:165–169

    Article  Google Scholar 

  • Liu P, Xue HZ, Zhou XY, Liu MJ, Mao YM, Zhou JY (2004) Study on the biological basis of pollination in Chinese jujube (Zizyphus jujuba) and wild jujube (Z. spinosa). J Fruit Sci 21:224–228

    Google Scholar 

  • Lloyd GB, McCown BH (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot culture. Comb Proc Int Plant Prop Soc 30:421–427

    Google Scholar 

  • Mathur N, Ramawat KG, Nandwani D (1995) Rapid in vitro multiplication of jujube through mature stem explants. Plant Cell Tissue Organ Cult 43:75–77

    Article  Google Scholar 

  • Matsuta N, Hirabayashi T (1989) Embryogenic cell lines from somatic embryos of grape (Vitis vinifera L.). Plant Cell Rep 7:684–687

    Google Scholar 

  • Molina DM, Aponte ME, Cortina H, Moreno G (2002) The effect of genotype and explant age on somatic embryogenesis of coffee. Plant Cell Tissue Organ Cult 71:117–123

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275

    CAS  Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404

    Google Scholar 

  • Sanford JC (1983) Ploidy manipulations. In: Moore JN, Janick J (eds) Methods in fruit breeding. Purdue University Press, West Lafayette, pp 100–123

    Google Scholar 

  • Shi XX, Du GQ, Gao Y, Ma BK, Xiao JH, Jia JH (1999) High adventitious shoot regeneration from leaves in vitro of apple cultivars. J Fruit Sci 16:255–258

    Google Scholar 

  • Suezawa K, Matsuta N, Omura M, Yamaki S (1988) Plantlet formation from cell suspensions of kiwifruit (Actinidia chinensis Planch, var. chinensis). Sci Hortic 37:123–128

    Article  Google Scholar 

  • Van Altvorst AC, Koehorst HJJ, Bruinsma T, Jansen J, Custers J, De Jong J, Dons JJM (1992) Adventitious shoot formation from in vitro leaf explants of carnation (Dianthus caryophyllus L). Sci Hortic 51:223–235

    Article  Google Scholar 

  • Wang CS, Li JH, Wang YQ, Zhao M (1999) Advance of research on post-harvest physiology and storage of fresh jujube. J Fruit Sci 16:219–223

    Google Scholar 

  • Wang YZ (1996) Shoot tip culture of Zizyphus jujuba and plantlet formation. Plant Physiol Commun 32:26–27

    Google Scholar 

  • Xu HL, Cheng JX, Yu DH, Shao QL, Cui HW (2003a) Study on the in vitro multiplication of zhanhua winter jujube. Shandong For Sci Technol 5:29–30

    Google Scholar 

  • Xu JR, Li SY, Zhang CS (2003b) Zizyphus jujuba CV. Dongzao and difference with other late-ripe jujube cultivars. Hebei J For Orch Res 18:28–42

    Google Scholar 

  • Zuker A, Ahroni A, Shejtman H, Vainstein A (1997) Adventitious shoot regeneration from leaf explants of Gypsophila paniculata L. Plant Cell Rep 16:775–778

    Article  Google Scholar 

Download references

Acknowledgments

This project was supported by the National Natural Science Foundation of China (30300242), the China Postdoctoral Science Foundation and the Department of Science and Technology of Shandong

Author information

Affiliations

  1. School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan, 250100, P.R. China

    X. F. Gu & J. R. Zhang

Authors
  1. X. F. Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. R. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. R. Zhang.

Additional information

Communicated by S.A. Merkle

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Gu, X.F., Zhang, J.R. An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants. Plant Cell Rep 23, 775–779 (2005). https://doi.org/10.1007/s00299-005-0920-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-005-0920-5

Keywords

  • Winter jujube (Zizyphus jujuba Mill.)
  • Adventitious shoot
  • Leaf explants
  • Thidiazuron