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Plant regeneration from callus cultures derived from mature zygotic embryos in white pine (Pinus strobus L.)

  • Cell Biology and Morphogenesis
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Abstract

Plant regeneration via adventitious shoot organogenesis from callus cultures initiated from mature embryos in white pine (Pinus strobus L.) was achieved in this study. Callus cultures were induced from mature embryos cultured on PS medium supplemented with 2,4-dichlorophenoxyacetic acid, α-naphthaleneacetic acid, or indole-3-acetic acid. Adventitious shoot regeneration from callus cultures was induced on medium containing 2 μM indole-3-butyric acid (IBA) and 3–12 μM N6-benzylaminopurine, thidiazuron (TDZ), or 6-(γ,γ-dimethylallylamino) purine. Sucrose was the most suitable sugar for adventitious shoot organogenesis in white pine. Shoot organogenesis was improved by treatment at 4°C for 6 weeks. The frequency of adventitious shoot formation increased when 0.1 mM putrescine was added to basal medium supplemented with 6 μM TDZ and 2 μM IBA. Putrescine improved adventitious shoot organogenesis by decreasing lipid peroxidation. These findings provide useful information on adventitious shoot organogenesis and may be valuable to genetic transformation in white pine.

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Fig. 1a–c
Fig. 2a–I
Fig. 3a–I

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Abbreviations

BA:

N6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

CH:

Casein enzymatic hydrosylate

IAA:

Indole-3-acetic acid

2iP:

6-(γ,γDimethylallylamino) purine

IBA:

Indole-3-butyric acid

NAA:

α-Naphthaleneacetic acid

TDZ:

Thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-ylurea)

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Acknowledgements

The authors are grateful to Nicki Whitley, Nicole Bloom, Tinya DelaGarza, Ambrosia Yarn, and Lonnie Gonsalves for their support in isolating mature embryos from seeds for this study.

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Authors and Affiliations

  1. Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC, 27858-4353, USA

    Wei Tang & Ronald J. Newton

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  1. Wei Tang
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  2. Ronald J. Newton
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Correspondence to Wei Tang.

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Communicated by P.P. Kumar

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Tang, W., Newton, R.J. Plant regeneration from callus cultures derived from mature zygotic embryos in white pine (Pinus strobus L.). Plant Cell Rep 24, 1–9 (2005). https://doi.org/10.1007/s00299-005-0914-3

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  • DOI: https://doi.org/10.1007/s00299-005-0914-3

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