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Mitotic instability in wheat×Thinopyrum ponticum derivatives revealed by chromosome counting, nuclear DNA content and histone H3 phosphorylation pattern

  • Genetics and Genomics
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Abstract

To evaluate the mitotic stability of Triticum aestivum×Thinopyrum ponticum derivatives (BC2F7 and BC2F5 doubled haploids), chromosome counting by both conventional and immunostaining techniques, and measurement of DNA content were performed. The wheat progenitor line, PF 839197, the wheat recurrent parent CEP 19 and the control Chinese Spring were also investigated. In the hybrid derivatives, chromosome number ranged from 2n=36 to 60, with a predominance of chromosome numbers higher than 2n=42, that was confirmed by determination of nuclear DNA content. Chinese Spring’ and PF 839197 were stable, but CEP 19 showed chromosome number variation (20%). Analyses of non-pretreated cells revealed the presence of anaphase bridges, lagging chromatids, chromosome fragments and micronuclei. Immunostaining with an antibody recognizing histone H3 phosphorylated showed dicentric chromatids forming anaphase bridges and pericentromeric phosphorylation at centric chromosome fragments but not at lagging chromatids. The possible causes of the observed mitotic instability are discussed.

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References

  • Bennett MD, Smith JS (1976) Nuclear DNA amounts in angiosperms. Phil Trans Roy Soc Lond Ser B 274:227–274

    CAS  Google Scholar 

  • Cai X, Jones SS, Murray TD (1998a) Molecular characterization of Thinopyrum and wheat-Thinopyrum translocated chromosomes in wheat−Thinopyrum amphiploid. Chromosome Res 6:183–189

    Google Scholar 

  • Cai X, Murray TD, Jones SS (1998b) Identification and manipulation of Thinopyrum ponticum chromatin conferring resistance to Cephalosporium stripe disease and a perennial habit in wheat. In: Slinkard AE (ed) Proceedings of the 9th International Wheat Genetic Symposium, vol 2. University of Saskatchewan, Saskatoon, pp 29–31

  • Casals F, Cáceres M, Ruiz A (2003) The foldback-like transposon Galileo is involved in the generation of two different natural chromosomal inversions of Drosophila buzzatii. Mol Biol Evol 20:674–685

    Google Scholar 

  • Chen Q, Conner RL, Laroche A, Thomas JB (1998) Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization. Genome 41:580–586

    Google Scholar 

  • Chen Q, Jahier J, Cauderon Y (1992) Production and cytogenetic analysis of BC1, BC2, and BC3 progenies of an intergeneric hybrid between Triticum aestivum (L.) Thell and tetraploid Agropyron cristatum (L.). Gaertn Theor Appl Genet 84:698–703

    Google Scholar 

  • Comai L (2000) Genetic and epigenetic interactions in allopolyploid plants. Plant Mol Biol 43:387–399

    Google Scholar 

  • Chrząstek M (2003) Cytogenetic stability of wheat lines (Triticum aestivum L.) with added and substituted chromosomes of rye (Secale cereale L.). Acta Biol Cracovesiana Ser Bot 45:117–126

    Google Scholar 

  • Ellneskog-Staam P, Merker A (2002) Chromosome composition, stability and fertility of alloploids between Triticum turgidum var. carthlicum and Thinopyrum junceiforme. Hereditas 136:59–65

    Google Scholar 

  • Finch RA, Bennett MD (1983) The mechanism of somatic chromosome elimination in Hordeum. In: Brandham PE, Bennett MD (eds) Proceedings of the 2nd Kew Chromosome Conference. Allen & Unwin, London, pp 147–154

  • Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87

    Google Scholar 

  • Gill KS, Arumuganathan K, Lee J-H (1999) Isolating individual wheat (Triticum aestivum) chromosome arms by flow cytometric analysis of ditelosomic lines. Theor Appl Genet 98:1248–1252

    Google Scholar 

  • Greilhuber J, Ebert I (1994) Genome size variation in Pisum sativum. Genome 37:646–655

    Google Scholar 

  • Guerra MS (1983) O uso do Giemsa na citogenética vegetal: comparação entre a coloração simples e o bandeamento. Ci Cult 35:190–193

    Google Scholar 

  • Guerra MS (1999) Haematoxylin: a simple multiple-use dye for chromosome analysis. Genet Mol Biol 22:77–80

    Google Scholar 

  • Guerra MS, Moraes-Fernandes MIB (1977) Somatic instability in the Brazilian semi-dwarf wheat IAS 54. Can J Genet Cytol 19:225–230

    Google Scholar 

  • Houben A, Wako T, Furushima-Shimogawara R, Presting G, Künzel G, Schubert I, Fukui K (1999) The cell cycle dependent phosphorylation of histone H3 is correlated with the condensation of plant mitotic chromosomes. Plant J 18:675–679

    Article  CAS  PubMed  Google Scholar 

  • Jauhar PP (1993) Alien gene transfer and genetic enrichment of bread wheat. In: Damania AB (ed) Biodiversity and wheat improvement. Wiley, Chichester, pp 103–119

    Google Scholar 

  • Jauhar PP (1995) Meiosis and fertility of F1 hybrids between hexaploid bread wheat and decaploid tall wheatgrass (Thinopyrum ponticum). Theor Appl Genet 90:865–871

    Google Scholar 

  • Jauhar PP, Peterson TS (2001) Hybrids between durum wheat and Thinopyrum junceiforme: Prospects for breeding for scab resistance. Euphytica 118:127–136

    Google Scholar 

  • Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212

    Google Scholar 

  • Jones N (2005) McClintock’s controlling elements: the full story. In: Puertas M, Naranjo T (eds) Cytogenetic and genome research. Special issue on plant cytogenetics, vol 109. Karger, Basel pp 90–103

    Google Scholar 

  • Kaszás E, Cande WZ (2000) Phosphorylation of histone H3 is correlated with changes in the maintenance of sister chromatid cohesion during meiosis in maize, rather than the condensation of the chromatin. J Cell Sci 113:3217–3226

    CAS  PubMed  Google Scholar 

  • Koba T, Handa T, Shimada T (1991) Efficient production of wheat-barley hybrids and preferential elimination of barley chromosomes. Theor Appl Genet 81:285–292

    Google Scholar 

  • Kubaláková M, Vrána J, Číhalíková J, Šimková H, Doležel J (2002) Flow karyotyping and chromosome sorting and chromosome sorting in bread wheat (Triticum aestivum L.). Theor Appl Genet 104:1362–1372

    Article  PubMed  Google Scholar 

  • Laurie DA, Bennett MD (1989) The timing of chromosome elimination wheat×maize crosses. Genome 32:953–961

    Google Scholar 

  • Lee J-H, Yen Y, Arumuganathan K, Baenzinger PS (1997) DNA content of wheat monosomics at intherphase estimated by flow cytometry. Theor Appl Genet 95:1300–1304

    Article  CAS  Google Scholar 

  • Manzareno S, Arana P, Puertas MJ, Houben A (2000) The chromosomal distribution of phosphorylated histone H3 differs between plants and animals at meiosis. Chromosoma 109:308–317

    CAS  PubMed  Google Scholar 

  • McIntosh RA, Hart GE, Devos KM, Gale MD, Rogers WJ (1998) Patogenic disease/pest reaction. In: McIntosh RA, Hart GE, Devos KM, Gale MD, Rogers WJ (eds) Proceedings of the 9th International Wheat Genetics Symposium. University of Saskatchewan, Saskatoon, pp 129–139

  • Page DR, Köhler C, Costa-Nunes JA, Baroux C, Moore JM (2004) Intrachromosomal excision of a hybrid Ds element induces large genomic deletions in Arabidopsis. PNAS 101:1969–1974

    Google Scholar 

  • Pfosser M, Amon A, Lafferty J, Heberle-Bors E, Lelley T (1995) Gain or loss of single chromosomes in wheat-rye addition lines and in 6x triticale detected by flow cytometry. Plant Breed 114:555–557

    Google Scholar 

  • Rayburn LA, Wetzel JB, Baligar VC (2002) Mitotic analysis of sticky chromosomes in aluminum tolerant and susceptible wheat lines grown in soils of differing aluminum saturation. Euphytica 127:193–199

    Google Scholar 

  • Sharma HC, Gill BS (1983) New hybrids between Agropyron and wheat. 2. Production, morphology and cytogenetic analysis of F1 hybrids and backcross derivatives. Theor Appl Genet 66:111–121

    Google Scholar 

  • Sybenga J (1992) Cytogenetics in plant breeding. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Vrána J, Kubaláková M, Šimková H, Číhalíková J, Lysák MA, Doležel J (2000) Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.). Genetics 156:2033–2041

    PubMed  Google Scholar 

  • Yang Q, Huang XT, Geng ZH,Yu XD (2002) Localization of phosphorylated histone H3 at mitosis and meiosis in wheat. Acta Bot Sin 44:1403–1408

    Google Scholar 

  • Zhang J-Y, Li X-M, Wang RR-C, Cortes A, Rosas A, Mujeeb-Kazi A (2002) Molecular cytogenetic characterization of Eb-genome chromosomes in Thinopyrum bessarabicum disomic addition lines of bread wheat. Int J Plant Sci 163:167–174

    Google Scholar 

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Acknowledgements

This work was supported by grants and fellowships from the Conselho Nacional de Desenvolvimento Científico e Tecnológico and the Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco. The authors are very grateful to Dilma Angra for helpful suggestions and M. J. Puertas acknowledges financial support for travel from the Complutense University of Madrid.

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Authors and Affiliations

  1. Departamento de Botânica, CCB, Universidade Federal de Pernambuco, Rua Nelson Chaves s/n, Cidade Universitária, 50670-420, Recife, Pernambuco, Brazil

    A. C. Brasileiro-Vidal & M. Guerra

  2. Centro Nacional de Pesquisa de Trigo, Empresa Brasileira de Pesquisa Agropecuária, Passo Fundo, Rio Grande do Sul, Brazil

    S. Brammer, A. C. Zanatta & A. Prestes

  3. Departamento de Genética, Facultad de Biología, Universidad Complutense, Madrid, Spain

    M. J. Puertas

  4. Instituto de Ciências Biológicas, Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil

    M. I. B. Moraes-Fernandes

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  1. A. C. Brasileiro-Vidal
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  2. S. Brammer
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  3. M. J. Puertas
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  4. A. C. Zanatta
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  5. A. Prestes
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  6. M. I. B. Moraes-Fernandes
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  7. M. Guerra
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Correspondence to A. C. Brasileiro-Vidal.

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Brasileiro-Vidal, A.C., Brammer, S., Puertas, M.J. et al. Mitotic instability in wheat×Thinopyrum ponticum derivatives revealed by chromosome counting, nuclear DNA content and histone H3 phosphorylation pattern. Plant Cell Rep 24, 172–178 (2005). https://doi.org/10.1007/s00299-005-0913-4

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  • DOI: https://doi.org/10.1007/s00299-005-0913-4

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