Abstract
Physcomitrella patens is a model plant for studying gene function using a knockout strategy. To establish a proteome database for P. patens, we resolved over 1,500 soluble proteins from gametophore and protonema tissues by two-dimensional electrophoresis (2-DE) and obtained peptide mass fingerprints (PMFs) by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Using expressed sequence tags (ESTs), we were able to predict the identities of 90 protein spots. Most of these were related to energy or primary metabolism. Comparative proteome analysis was used to identify proteins specific for each of the tissue types. One of these was a metallothionein type-2 (PpMT2) protein that was highly upregulated in gametophore tissue. PpMT2 was induced in both the gametophore and protonema following culture on solid media and in response to various abiotic stresses such as copper, cadmium, cold, indole-3-acetic acid, and ethylene. We suggest that PpMT2 is not only involved in metal binding and detoxification, but also in many biological aspects as a metal messenger or a protein with additional functions.
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Acknowledgements
This work was supported by a grant from the BioGreen21 Program of the Rural Development Administration (to J.S.S.) and by the Korea Research Foundation Grant (MOEHRD, Basic Research Promotion Fund) (to S.H.C., no. M01-2004-000-10317-0). Sung Hyun Cho and Quoc Truong Hoang contributed equally to this study
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Cho, S.H., Hoang, Q.T., Kim, Y.Y. et al. Proteome analysis of gametophores identified a metallothionein involved in various abiotic stress responses in Physcomitrella patens . Plant Cell Rep 25, 475–488 (2006). https://doi.org/10.1007/s00299-005-0079-0
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DOI: https://doi.org/10.1007/s00299-005-0079-0