Plant Cell Reports

, Volume 25, Issue 4, pp 257–264 | Cite as

Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

  • L. Cistué
  • M. Soriano
  • A. M. Castillo
  • M. P. Vallés
  • J. M. Sanz
  • B. Echávarri
Cell Biology and Morphogenesis

Abstract

The objective of this work was to produce doubled haploid plants from durum wheat through the induction of androgenesis. A microspore culture technique was developed and used to produce fertile doubled haploid plants of agronomic interest. Five cultivars, one selected line, plus a collection of 20 F1 crosses between different genotypes of high breeding value were used. Studies on several factors such as pre-treatments and media components were carried out in order to develop a protocol to regenerate green haploid plantlets. Anthers were pre-treated in 0.7 M mannitol. Microspores, from anther maceration, were plated on a C17 induction culture medium with ovary co-culture. The optimum regeneration medium J25–8 was used. From 35 microspore isolations, 407 green plantlets were obtained. With this technique mature embryos were obtained. Green plants were regenerated from all genotypes used and approximately 67% of them were spontaneously doubled haploids. Some haploids and a very few polyploids plants were obtained. From the 407 plants, 275 were completely fertile and gave enough seeds to be assayed in the field. This protocol could be used complementary to or instead of the intergeneric crossing with maize as an economically feasible method to obtain doubled haploids from most durum wheat genotypes.

Keywords

Doubled haploid Microspore culture Durum wheat Triticum turgidum 

Abbreviations

DH

Doubled haploid

2,4-D

2,4-dic-hlorophenoxyacetic acid

BAP

6-Benzylaminopurine

KIN

6-Furfurylaminopurine

NAA

Naphthalene acetic acid

PAA

Phenylacetic acid

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • L. Cistué
    • 1
  • M. Soriano
    • 1
  • A. M. Castillo
    • 1
  • M. P. Vallés
    • 1
  • J. M. Sanz
    • 1
  • B. Echávarri
    • 1
  1. 1.Departamento de Genética y Producción VegetalEstación Experimental de Aula Dei, Consejo Superior de Investigaciones CientíficasZaragozaSpain

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