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Xanthan gum: an economical substitute for agar in plant tissue culture media

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Xanthan gum, a microbial desiccation-resistant polysaccharide prepared commercially by aerobic submerged fermentation from Xanthomonas campestris, has been successfully used as a solidifying agent for plant tissue culture media. Its suitability as a substitute to agar was demonstrated for in vitro seed germination, caulogenesis and rhizogenesis of Albizzia lebbeck, androgenesis in anther cultures of Datura innoxia, and somatic embryogenesis in callus cultures of Calliandra tweedii. Culture media used for eliciting these morphogenic responses were gelled with either 1% xanthan gum or 0.9% agar. Xanthan gum, like agar, supported all these responses.

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Fig. 1

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6-Benzylamino purine


Murashige and Skoog's basal medium

B5 :

Gamborg's basal medium


  • Babbar SB, Jain N (1998) ‘Isubgol’ as an alternative gelling agent for plant tissue culture media. Plant Cell Rep 17:318–322

    Article  CAS  Google Scholar 

  • Babbar SB, Jain R, Walia N (2005) Guar gum as a gelling agent for plant tissue culture media. In Vitro Cell Dev Biol–Plant 41 (in press)

  • Creager JG, Black JG, Davison VE (1990) Microbiology, principles and applications. Prentice Hall, New Jersey

    Google Scholar 

  • Eyre MJ, Caswell SC (1991) Sterile culture of Rotylenchulus reniformis on tomato root with gellan gum as a supporting medium. J Nematol 23:229–231

    Google Scholar 

  • Gamborg OL, Muller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Gupta SC, Babbar SB (1980) Enhancement of plantlets formation in anther cultures of Datura metel L. by pre-chilling of buds. Z Pflanzenphysiol 96:465–470

    Google Scholar 

  • Henderson WE, Kinneresley AM (1988) Corn starch as an alternative gelling agent for plant tissue culture. Plant Cell Tissue Organ Cult 15:17–22

    Article  Google Scholar 

  • Jain N, Babbar SB (2002) Gum katira—a cheap gelling agent for plant tissue culture media. Plant Cell Tissue Organ Cult 71:223–229

    Article  CAS  Google Scholar 

  • Lachke A (2004) Xanthan—a versatile gum. Resonance 9:25–33

    Google Scholar 

  • McLachlan (1985) Macroalgae (seaweeds): industrial resources and their utilization. Plant and Soil 89:137–157

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with the tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404

    Google Scholar 

  • Pasqualetto PL, Zimmerman RH, Fordham IM (1988) The influence of cation and gelling agent concentrations on vitrification of apple cultivars in vitro. Plant Cell Tissue Organ Cult 14:31–40

    Article  CAS  Google Scholar 

  • Sorvari S, Toldi O, Ahanen K, Viinämaki T, Hakonen T, Tahvonen R (1997) Using polysaccharides and galactomannans as gelling agents in capsule formation of artificial seeds. J Am Soc Hortic Sci 122:878–883

    CAS  Google Scholar 

  • Trembley L, Trembley FM (1991) Effects of gelling agents, ammonium nitrate and light on the development of Picea mariana (Mill.) B.S.P. (black spruce) and Picea rubens Sarg. (red spruce) somatic embryos. Plant Sci 77:233–242

    Article  Google Scholar 

  • White PR (1939) Controlled differentiation in plant tissue culture. Bull Torrey Bot Club 66:507–513

    Article  Google Scholar 

  • Zimmerman RH, Bhardwaj SV, Fordham I (1995) Use of starch-gelled medium for plant tissue culture of some fruit crops. Plant Cell Tissue Organ Cult 43:207–213

    Google Scholar 

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Correspondence to S. B. Babbar.

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Communicated by G.C. Phillips

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Jain, R., Babbar, S.B. Xanthan gum: an economical substitute for agar in plant tissue culture media. Plant Cell Rep 25, 81–84 (2006).

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