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Analysis of mycorrhizal associations formed by Cistus incanus transformed root clones with Terfezia boudieri isolates

Plant Cell Reports volume 25, Article number: 62 (2006) Cite this article

Abstract

One clone (M-2), out of several Agrobacterium rhizogenes transformed root clones of Cistus incanus, formed ecto- or endomycorrhiza in vitro with two isolates of Terfezia boudieri collected in Israel. All other clone-fungal isolate combinations formed ectomycorrhiza. The endomycorrhiza-forming isolate secreted smaller amounts of auxin than an ectomycorrhiza-forming isolate. Addition of 2,4-dichlorophenoxyacetic acid (2,4-D) led to ectomycorrhiza formation by the M-2 clone on low P medium. Endomycorrhizas were formed by both M-2 and a control clone with the same T. boudieri isolates on high P medium with 2,4-D. The M-2 clone of C. incanus exhibited greater sensitivity to exogenous auxins (IAA and 2,4-D) than other clones, and clonal sensitivity to auxin was increased tenfold under low P conditions. Results are discussed in relation to phosphate and auxin influence on T. boudieri–C. incanus interaction.

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References

  • Allen MJ (1992) Mycorrhizal functioning: an integrative plant–fungal process. Routledge, Chapman & Hall, Inc., New York

    Google Scholar 

  • Alsheikh A (1985) Mycorrhizae of annual Helianthemum species formed with desert truffles. In: Molina R (ed) Proceedings of the 6th North American Conference on Mycorrhizae, June 25–29, 1984. Oregon State University, Forest Research Laboratory, Corvallis, Oregon. p 471

  • Awameh MS (1981) The response of Helianthemum salicifolium and H. ledifolium to the infection by the desert truffle Terfezia boudieri. Mushroom science. XI. Part II. In: Proceedings of the 11th International Congress on the Cultivation of Edible Fungi, Sydney, Australia, pp 843–583

  • Barker SJ, Tagu D (2000) The roles of auxins and cytokinins in mycorrhizal symbiosis. J Plant Growth Regul 19:144–154

    PubMed  CAS  Google Scholar 

  • Becard G, Fortin JA (1988) Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA transformed roots. New Phytol 108:211–218

    Article  CAS  Google Scholar 

  • Bohrer G, Kagan-Zur V, Roth-Bejerano N, Ward D (2001) Effects of environmental variables on vesicular-arbuscular mycorrhizal abundance in wild populations of Vangueria infausta. J Veget Sci 12:279–288

    Article  Google Scholar 

  • Bonfante PF, Fontana A (1973) Sulla nutrizione del mycelia di Tuber melanosporum Vitt in coltura. Atti della Accademia delle Scienze di Torino 107:713–714

    Google Scholar 

  • Brunner I, Scheidegger C (1994) Effects of high nitrogen concentrations on ectomycorrhizal structure and growth of seedlings of Picea abies (L.) Karst. New Phytol 129:83–95

    Article  Google Scholar 

  • Chevalier, Riousset L, Dexheimer J, Dupré C (1984) Synthèse mycorhizienne entre Terfezia leptoderma Tul. et diverses Cistacées. Agronomie 4:210–211

    Google Scholar 

  • Cohen BA, Amsellem Z, Maor R, Sharon A, Gressel J (2002) Transgenically enhanced expression of indole-3 acetic acid confers hypervirulence to plant pathogens. Phytopathology 92(6):590–596

    Article  CAS  PubMed  Google Scholar 

  • Dexheimer J, Gerard J, Leduc JP, Chevalier G (1985) Étude ultrastructurale comparée des associations symbiotiques mycorhiziennes Heliathemum salicifolium–Terfezia claveryi et Heliathemum salicifolium–Terfezia leptoderma. Can J Bot 63(3):582–591

    Article  Google Scholar 

  • Fortas Z, Chevalier G (1992) Effet des conditions de culture sur la mycorhization de ľHelianthemum guttatum par trois espèces de terfez des genres Terfezia et Tirmania ďAlgérie. Can J Bot 70:2453–2460

    Google Scholar 

  • Gay G, Normand L, Marmeisse R, Sotta B, Debaud JC (1994) Auxin overproducer mutant of Hebeloma cylindrosporum Romagnési have increased mycorrhizal activity. New Phytol 128:645–657

    Article  CAS  Google Scholar 

  • Gea L, Normand L, Vian B, Gay G (1994) Structural aspects of ectomycorrhiza of Pinus pinaster (Ait.) Sol. formed by an IAA-overproducer mutant of Hebeloma cylindrosporum Romagnési. New Phytol 128:659–670

    Article  Google Scholar 

  • Giovannetti G, Fontana A (1982) Mycorrhizal synthesis between Cistaceae and Tuberaceae. New Phytol 92:533–537

    Article  Google Scholar 

  • Giovannetti G, Roth-Bejerano N, Zanini E, Kagan-Zur V (1994) Truffles and their cultivation. Hortic Rev 17:71–107

    Google Scholar 

  • Graham JH, Leonard RT, Menge JA (1981) Membrane-mediated decrease in root exudation responsible for phosphorus inhibition of vesicular arbuscular formation. Plant Physiol 68:548–552

    PubMed  CAS  Google Scholar 

  • Gutiérrez A, Morte A, Honrubia M (2003) Morphological characterization of the mycorrhiza formed by Helianthemum almeriense with Terfezia clavery Chatin and Picoa lefebvrei (Pat.) Maire. Mycorrhiza 13:299–307

    PubMed  Article  Google Scholar 

  • Harley JL, Smith SE (1983) Mycorrhizal symbiosis. Academic, London

    Google Scholar 

  • Haug S, Ritter T, Kottke I, Oberwinkler F (1992) Steigerung der Leistungsfähigkeit von Forstpflanzen (Fagus silvatica L. und Quercus robur L.) durch kontrollierte Mykorrhizierung. Allg Forst Jagdztg 163:72–79

    Google Scholar 

  • Hilbert JL, Martin F (1988) Regulation of gene expression in ectomycorrhizas. I. Protein changes and the presence of ectomycorrhiza-specific polypeptides in the Pisolithus-Eucalyptus symbiosis. New Phytol 110:339–346

    Article  CAS  Google Scholar 

  • Hoagland DR, Arnon DI (1950) The water culture method for plants without soil. Circular 374. University of California, Agriculture Experimental Station, Berkeley

    Google Scholar 

  • Joanin L (1984) Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids. Plasmid 12:91–102

    Article  Google Scholar 

  • Jouanin L, Guerche P, Pamboukjian N, Tourneur C, Cassa-Delbart F, Tourneur J (1987) Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4. Mol Gen Genet 206:387–392

    Article  CAS  Google Scholar 

  • Kagan-Zur V, Kuang J, Tabak S, Taylor F, Roth-Bejerano N (1999) Potential verification of a host plant for the desert truffle Terfezia pfeilii by molecular methods. Mycol Res 103(10):1270–1274

    Article  CAS  Google Scholar 

  • Kagan-Zur V, Raveh E, Lischinsky S, Roth-Bejerano N (1994) Helianthemum-Terfezia association is enhanced by lack of iron in the growth mediums. New Phytol 127:567–570

    Article  CAS  Google Scholar 

  • Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect vesicular-arbuscular mycorrhizas. Mycol Res 92:486–505

    Google Scholar 

  • López-Bucio J, Hernández-Abreu E, Sánchez-Caldéron L, Nieto-Jacobo MF, Simpson J, Herrera-Estrella L (2002) Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiol 129:244–256

    PubMed  Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473–497

    Article  CAS  Google Scholar 

  • Norman JE, Egger KN (1999) Molecular phylogenetic analysis of Peziza and related genera. Mycologia 91:820–829

    Article  CAS  Google Scholar 

  • Normanly J (1997) Auxin metabolism. Physiol Plantarum 100:431–442

    Article  CAS  Google Scholar 

  • Percudani R, Trevisi A, Zambonelli A, Ottonello S (1999) Molecular phylogeny of truffles (Pezizales: Terfeziaceae, Tuberaceae) derived from nuclear rDNA sequence analysis. Mol Phylogenet Evol 13:169–180

    PubMed  Article  CAS  Google Scholar 

  • Petit A, Tempe J (1978) Isolation of Agrobacterium Ti plasmid regulatory mutants. Mol Gen Genet 167:147–155

    Article  CAS  Google Scholar 

  • Raven PH, Evert RF, Eichhorn S (1999) Biology of plants, 6th edn. Freeman, New York

    Google Scholar 

  • Roth-Bejerano N, Livne D, Kagan-Zur V (1990) Helianthemum-Terfezia relations in different media. New Phytol 114:235–238

    Article  Google Scholar 

  • Schaerer S, Pilet P (1993) Quantification of indole-3-acetic acid in untransformed and Agrobacterium rhizogenes-transformed pea roots using gas chromatography mass spectrometry. Planta 189:55–59

    Article  CAS  Google Scholar 

  • Shen WH, Davioud E, David C, Barbier-Brygoo H, Tempé J, Guern J (1990) High sensitivity to auxin is a common feature of hairy roots. Plant Physiol 94:554–560

    PubMed  CAS  Google Scholar 

  • Shen WH, Petit A, Guern J, Tempé J (1988) Hairy roots are more sensitive to auxin than normal roots. Proc Natl Acad Sci USA 85:3417–3421

    PubMed  Article  CAS  Google Scholar 

  • Tanaka N, Fujikawa Y, Aly MAM, Saneoka H, Fujita K, Yamashita I (2001) Proliferation and rol gene expression in hairy root lines of Egyptian clover. Plant Cell Tiss Org 66:175–182

    Article  CAS  Google Scholar 

  • Thomson BD, Malajczuk N, Grove TS, Hardy GEStJ (1993) Improving the colonization capacity and effectiveness of ectomycorrhizal fungal cultures by association with a host plant and re-isolation. Mycol Res 97(7):839–844

    Article  Google Scholar 

  • Tonkin CM, Malajczuk N, McComb JA (1989) Ectomycorrhizal formation by microproprogated clones of Eucalyptus marginata inoculated with isolates of Pisolithus tinctorius. New Phytol 111:209–214

    Article  Google Scholar 

  • Wenkart S, Roth-Bsejerano N, Mills D, Kagan-Zur V (2001) Mycorrhizal associations between Tuber melanosporum mycelia and transformed roots of Cistus incanus. Plant Cell Rep 20:369–373

    Article  CAS  Google Scholar 

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Acknowledgment

We thank Dr. Zeev Wiesman for his advice and help in IAA analyses

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Affiliations

  1. Department of Life Sciences, Ben Gurion University, POB 653,, Beer Sheva, Israel

    M. Zaretsky & N. Roth-Bejerano

  2. The Institutes for Applied Research, Ben Gurion University, POB 653,, Beer Sheva, Israel

    V. Kagan-Zur & D. Mills

Authors
  1. M. Zaretsky
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  2. V. Kagan-Zur
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  3. D. Mills
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  4. N. Roth-Bejerano
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Corresponding author

Correspondence to N. Roth-Bejerano.

Additional information

Communicated by K. K. Kamo

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Zaretsky, M., Kagan-Zur, V., Mills, D. et al. Analysis of mycorrhizal associations formed by Cistus incanus transformed root clones with Terfezia boudieri isolates. Plant Cell Rep 25, 62 (2006). https://doi.org/10.1007/s00299-005-0035-z

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  • DOI: https://doi.org/10.1007/s00299-005-0035-z

Keywords

  • Auxin
  • Mycorrhiza
  • Phosphate
  • Terfezia
  • Transformed roots

Abbreviations

  • 2,4-D: 2,4-Dichlorophenoxyacetic acid
  • IAA: Indole 3-acetic acid