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Direct gene transfer in potato: A comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts

  • Genetic Transformation and Hybridization
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Abstract

Direct gene transfer methods in potato would facilitate the transfer of multiple genes and the manipulation of metabolic pathways in this species. In this study, up to 1.8 transformation events per shot (=0.5 per bombarded leaf) and 67.2 events per million protoplasts treated were obtained with particle bombardment and PEG-mediated direct DNA uptake, respectively. Limited disassociation of both HPT and GUS genes appeared to occur during the process of integration in only 19% of transformants. A large number of transformed potato plants with transgene expression at levels comparable to Agrobacterium-mediated transformation was obtained. High levels of GUS expression were only obtained in lines derived from PEG treatment. No correlation between the number of gene insertions and gene expression levels was found, suggesting that multiple insertions may have little or no effect on transgene expression.

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Acknowledgements

We extend our heartfelt gratitude to A. Morgese and P. Palmieri for their excellent technical assistance. This work was partially funded by the European Union 5th Framework Programme, project “The Plastid Factory” (QLK3-CT-1999-00692). This article is contribution number 53 from the Institute of Plant Genetics, Research Division of Portici

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Authors and Affiliations

  1. CNR-IGV, Institute of Plant Genetics, Res. Div. Portici, via Università 133, 80055, Portici (NA), Italy

    W. Craig, D. Gargano, N. Scotti & T. Cardi

  2. Plant Cell Biology Laboratory, Biology Department, National University of Ireland-Maynooth, Maynooth, Co., Kildare, Ireland

    T. T. Nguyen & P. J. Dix

  3. Plant Molecular Biology Laboratory, Smurfit Institute of Genetics, Trinity College, Lincoln Place Gate, Dublin 2, Ireland

    N. T. Lao & T. A. Kavanagh

Authors
  1. W. Craig
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  2. D. Gargano
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  3. N. Scotti
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  4. T. T. Nguyen
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  5. N. T. Lao
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  6. T. A. Kavanagh
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  7. P. J. Dix
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  8. T. Cardi
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Corresponding author

Correspondence to T. Cardi.

Additional information

Communicated by D. Dudits

W. Craig and D. Gargano contributed equally to this work

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Craig, W., Gargano, D., Scotti, N. et al. Direct gene transfer in potato: A comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts. Plant Cell Rep 24, 603–611 (2005). https://doi.org/10.1007/s00299-005-0018-0

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  • DOI: https://doi.org/10.1007/s00299-005-0018-0

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