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In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua


Tetraploid plants of Zizyphus jujuba Mill. cv. Zhanhua were obtained with in vitro colchicine treatment. Shoot tips from in vitro-grown plants were treated with five different concentrations of colchicine (0.01, 0.03, 0.05, 0.1, 0.3%) in liquid MS medium (Murashige and Skoog 1962), and shaken (100 rpm) at 25 °C in darkness for 24, 48, 72 or 96 h, respectively. Tetraploids were obtained at a frequency of over 3% by using 0.05% colchicine (48 h, 72 h) and 0.1% colchicine (24 h, 48 h) treatment as determined by flow cytometry. Cytological and morphological evidence confirmed the results of flow cytometric analysis. The chromosome number of diploid plants was 24 and that of tetraploid plants was 48. The stomata sizes of tetraploid plants were significantly larger than those of diploid plants, while the frequency of stomata were reduced significantly. Similarly, the chloroplast number of guard cells of tetraploid plants increased significantly. The selected tetraploid plants were grafted onto mature trees of Z. jujuba Mill. cv. Zhanhua in the field, resulted in thicker stems, rounder and succulent leaves, larger flowers and a delay in florescence time (3–4 days later) than diploid plants.

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BA :


GA 3 :

Gibberellic acid


Indole-3-acetic acid


Indole-3-butyric acid

MS :

Murashige and Skoog (1962) medium


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This research was supported by the National Natural Science Foundation of China (30300242), the project of Department of Science and Technology of Shandong and the China Postdoctoral Science Foundation

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Correspondence to J. R. Zhang.

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Communicated by P. Ozias-Akins

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Gu, X.F., Yang, A.F., Meng, H. et al. In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua. Plant Cell Rep 24, 671–676 (2005).

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  • Winter jujube
  • Colchicine
  • Tetraploid induction
  • Flow cytometry