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Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture

Plant Cell Reports volume 24, Article number: 691 (2006) Cite this article

An Erratum to this article was published on 04 July 2006

Abstract

The objective of this study was to improve induction of embryogenesis in wheat microspore culture in order to obtain a high number of regenerable embryos. The arabinogalactan (AG) Larcoll and the arabinogalactan-protein (AGP) from gum arabic were tested on two spring genotypes to see if they could increase microspore viability and induce embryogenesis in the microspore culture. Adding Larcoll significantly decreased microspore mortality in both genotypes regardless of the presence or absence of ovaries in the culture. Similarly, gum arabic had a strong effect on the number of embryos produced and regenerated green plants. In fact, by using only gum arabic we were able to obtain green plants from wheat microspore cultures without the presence of ovaries. In addition to preventing a high mortality rate of the cells, our results show that the induction of embryogenesis in wheat microspore cultures is strongly affected by the use of both AG or AGP.

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Abbreviations

AGs :

Arabinogalactan

AGPs :

Arabinogalactan-protein

DHs :

Doubled haploid

ELSs :

Embryo-like-structures

PCDs :

Programmed cell death

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Acknowledgements

The authors gratefully acknowledge funding provided by NSERC, Monsanto and the Ontario Ministry of Agriculture and Food

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Affiliations

  1. Department of Plant Agriculture, University of Guelph, Ontario, Canada, N1G 2W1

    Jocelyne Letarte, Ecaterina Simion, Mai Miner & Ken J. Kasha

Authors
  1. Jocelyne Letarte
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  2. Ecaterina Simion
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  3. Mai Miner
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  4. Ken J. Kasha
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Corresponding author

Correspondence to Ken J. Kasha.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-006-0180-z

Communicated by M. C. Jordan

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Letarte, J., Simion, E., Miner, M. et al. Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. Plant Cell Rep 24, 691 (2006). https://doi.org/10.1007/s00299-005-0013-5

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  • DOI: https://doi.org/10.1007/s00299-005-0013-5

Keywords

  • Gum arabic
  • Haploid
  • Larcoll
  • Polysaccharide
  • Programmed cell death
  • Wheat doubled haploids