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Micropropagation of squill (Charybdis numidica) through nodule culture

  • Cell Biology and Morphogenesis
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Abstract

A micropropagation protocol for squill (Charybdis numidica, Hyacinthaceae) was developed using nodule culture. Nodule formation on leaf sections was induced in liquid Murashige and Skoog (MS) medium supplemented with 20 μM N6-benzylaminopurine (BA) under dark conditions. Nodules were cultured on semi-solid MS medium with factorial combinations of BA (0–40 μM) and α-naphthaleneacetic acid (NAA) (0–10 μM) under continuous light. Shoot regeneration from nodules occurred at varying degrees on all media. The highest number of shoots was formed on medium containing 2.5 μM NAA and 20 μM BA, while the maximum number of regenerated bulblets per gram nodule was induced on culture medium supplemented with 2.5 μM NAA alone. Regenerated shoots were successfully rooted at approximately 92% on semi-solid MS medium supplemented with 10 μM indole-3-acetic acid (IAA). Plantlets could be hardened and grew well after transfer to the greenhouse. Chemical analyses showed consistent bufadienolide patterns from cloned plantlets and the mother plant.

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Fig. 1a–e

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Abbreviations

BA:

N6-Benzylaminopurine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog medium

NAA:

α-Naphthaleneacetic acid

TLC:

Thin layer chromatography

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Acknowledgements

A. Kongbangkerd is indebted to the Austrian Academic Exchange Service for the award of a scholarship. We thank Dr. S. Prinz for carrying out the chemical analyses

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Authors and Affiliations

  1. Institute of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria

    A. Kongbangkerd, A. Köpf, P. Allacher, C. Wawrosch & B. Kopp

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  1. A. Kongbangkerd
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  2. A. Köpf
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  3. P. Allacher
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  4. C. Wawrosch
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  5. B. Kopp
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Corresponding author

Correspondence to B. Kopp.

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Communicated by W. Barz

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Kongbangkerd, A., Köpf, A., Allacher, P. et al. Micropropagation of squill (Charybdis numidica) through nodule culture. Plant Cell Rep 23, 673–677 (2005). https://doi.org/10.1007/s00299-004-0907-7

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  • DOI: https://doi.org/10.1007/s00299-004-0907-7

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