FPF1 transgene leads to altered flowering time and root development in rice

Abstract

AtFPF1 (FLOWERING PROMOTING FACTOR 1) is a gene that promotes flowering in Arabidopsis. An expression vector containing AtFPF1 driven by a Ubi-1 promoter was constructed. The gene was introduced into rice callus by Agrobacterium-mediated transformation and fertile plants were obtained. The presence of AtFPF1 in rice plants was confirmed by PCR, Southern and Northern blot analyses, as well as by β-glucuronidase assay. The results showed that, as in Arabidopsis, AtFPF1 reduced flowering time in rice. Furthermore, introduction of AtFPF1 enhanced adventitious root formation but inhibited root growth in rice during the seedling stage. The results suggest that AtFPF1 promotes flowering time in both dicots and monocots, and plays a role in the initiation of adventitious roots in rice.

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Abbreviations

6-BA:

6-Benzyladenine

GUS:

β-Glucuronidase

Hyg:

Hygromycin

KT:

Kinetin

NAA:

α-Napthylacetic acid

ZT:

Zeatin

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Acknowledgements

The authors are grateful to Dr. S. Melzer, Swiss Federal Institute of Technology, Institute for Plant Sciences, Zurich, Switzerland, for his kind gift of the plasmid with AtFPF1. We also thank Dr. C.B. Chen, Penn State University, Dr. L.N. Tian, Agriculture & Agri-food Canada, and Dr. C Larue, University of Missouri for their critical reading of the manuscript. This project was supported by the Major State Basic Research Program of P.R. China (G19990116), partially by the National Nature Science Foundation of China (NSFC, 30270143), and the Innovation Grant of CAS, as well as the State High-Tech Project (2001AA222281).

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Correspondence to Kang Chong.

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Ming-Li Xu and Jia-Fu Jiang contributed equally to this work

Communicated by P. Lakshmanan

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Xu, M., Jiang, J., Ge, L. et al. FPF1 transgene leads to altered flowering time and root development in rice. Plant Cell Rep 24, 79–85 (2005). https://doi.org/10.1007/s00299-004-0906-8

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Keywords

  • AtFPF1
  • Flowering
  • Rice
  • Root development
  • Transgenic plant