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Evaluation of 12 β-lactam antibiotics for Agrobacterium-mediated transformation through in planta antibacterial activities and phytotoxicities

  • Genetic Transformation and Hybridization
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Abstract

The antibacterial activities of 12 β-lactam antibiotics against Agrobacterium tumefaciens strains LBA4404 and EHA101 living in tobacco (Nicotiana tabacum L.) leaf tissues, and their phytotoxicities to tobacco leaf tissues were evaluated. All β-lactams at minimum bactericidal concentration (MBC) or higher showed weak bactericidal activities against agrobacteria persisting in tobacco leaf tissues. The β-lactams evaluated were classified into two major groups according to their inhibitory effect on shoot regeneration of tobacco leaf tissues: (1) highly phytotoxic drugs, and (2) moderately phytotoxic drugs. According to these results, suitable kind and concentration of β-lactam antibiotics were evaluated for Agrobacterium-mediated transformation.

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References

  • Aida R, Hirose Y, Kishimoto S, Shibata M (1999) Agrobacterium tumefaciens-mediated transformation of Cyclamen persicum Mill. Plant Sci 148:1–7

    Article  CAS  Google Scholar 

  • Asbel LE, Levison ME (2000) Cephalosporins, carbapenems, and monobactams. Infect Dis Clin North Am 14:435–447

    CAS  PubMed  Google Scholar 

  • Barrett C, Cassells AC (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. pelargonii (Brown) from Pelargonium × domesticum cv. ‘Grand Slam’ explant in vitro. Plant Cell Tissue Organ Cult 36:169–175

    CAS  Google Scholar 

  • Barrett C, Cobb E, McNicol R, Lyon G (1997) A risk assessment study of plant genetic transformation using Agrobacterium and implications for analysis of transgenic plants. Plant Cell Tissue Organ Cult 47:135–144

    Google Scholar 

  • Cooke DL, Waites WM, Leifert C (1992) Effect of Agrobacterium tumefaciens, Erwinia caratovora, Pseudomonas syringae and Xanthomonas campestris on plant tissue cultures of Aster, Cheiranthus, Delphinium, Iris, and Rosa; disease development in vivo as a result of latent infection in vitro. Plant Dis Prot 99:469–481

    Google Scholar 

  • Craig WA, Ebert SC (1991) Killing and regrowth of bacteria in in vitro: a review. Pediatr Infect Dis J S74:63–70

    Google Scholar 

  • Demain AL, Elander RP (1999) The β-lactam antibiotics: past, present, and future. Antonie Van Leeuwenhoek 75:5–19

    Article  CAS  PubMed  Google Scholar 

  • Enomoto S, Itoh H, Ohshima M, Ohashi Y (1990) Induced expression of a chimeric gene construct in transgenic lettuce plants using tobacco pathogenesis-related protein gene promoter region. Plant Cell Rep 9:6–9

    Article  CAS  Google Scholar 

  • Falkiner FR (1988) Strategy of the selection of antibiotics for use against common bacterial pathogens and endophytes of plants. Acta Hortic 225:53–56

    Google Scholar 

  • Falkiner FR (1990) The criteria for choosing an antibiotic for control of bacteria in plant tissue culture. Int Assoc Plant Tissue Cult Newsl 60:13–23

    Google Scholar 

  • Frary A, Earle ED (1996) An examination of factors affecting the efficiency of Agrobacterium-mediated transformation of tomato. Plant Cell Rep 16:235–240

    Article  CAS  Google Scholar 

  • Fukasawa M, Sumita Y, Tada E, Okuda T (1992) In vitro antibacterial activity of meropenem. Chemotherapy 40(S-1):74–89

    CAS  Google Scholar 

  • Ghuysen J-M (1997) Penicillin-binding proteins. Wall peptidoglycan assembly and resistance to penicillin: facts, doubts and hopes. Int J Antimicrob Agents 8:45–60

    Article  CAS  Google Scholar 

  • Hammerschlag FA, Zimmerman RH, Yadava UL, Hunsucker S, Gercheva P (1997) Effect of antibiotics and exposure to an acidified medium on the elimination of Agrobacterium tumefaciens from apple leaf explants and on shoot regeneration. J Am Soc Hortic Sci 122:758–763

    CAS  Google Scholar 

  • Hayward AC (1974) Latent infections by bacteria. Annu Rev Phytopathol 12:87–97

    Article  Google Scholar 

  • Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) A binary vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303:179–180

    CAS  Google Scholar 

  • Hood EE, Helmer GC, Fraley RT, Chilton M-D (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside the T-DNA. J Bacteriol 168:1291–1301

    CAS  PubMed  Google Scholar 

  • Matzk A, Mantell S, Schiemann J (1996) Localization of persisting agrobacteria in transgenic plants. Mol Plant Microbe Interact 9:373–381

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Nauerby B, Billing K, Wyndaele R (1997) Influence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci 123:169–177

    Article  CAS  Google Scholar 

  • Ogawa Y, Mii M (2004) Screening for highly active β-lactam antibiotics against Agrobacterium tumefaciens. Arch Microbiol 181:331–336

    Article  CAS  PubMed  Google Scholar 

  • Okkels FT, Pedersen MG (1988) The toxicity to plant tissue and to Agrobacterium tumefaciens of some antibiotics. Acta Hortic 225:199–207

    Google Scholar 

  • Pollock K, Barfield DG, Shields R (1983) The toxicity of antibiotics to plant cell culture. Plant Cell Rep 2:36–39

    CAS  Google Scholar 

  • Schroeder HE, Schotz AH, Wardley-Ricardson T, Spencer D, Higgins TJV (1993) Transformation and regeneration of pea (Pisum sativum L.). Plant Physiol 101:751–757

    Google Scholar 

  • Shackelford NJ, Chlan CA (1996) Identification of antibiotics that are effective in eliminating Agrobacterium tumefaciens. Plant Mol Biol Rep 14:50–57

    CAS  Google Scholar 

  • van der Hoeven C, Dietz A, Landsmann J (1991) Agrobacteria shown to reside in transgenic plants. Nachrichtenbl Dtsch Pflanzenschutzdienstes 43:249–251

    Google Scholar 

  • Vergauwe A, Van Geldre E, Inzé D, Van Montagu M, Van den Eeckhout E (1996) The use of amoxicillin and ticarcillin in combination with a β-lactamase inhibitor as decontaminating agents in the Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. J Biotechnol 52:89–95

    Article  CAS  Google Scholar 

  • Young PM, Hutchins AS, Canfield ML (1984) Use of antibiotics to control bacteria in shoot cultures of woody plants. Plant Sci Lett 34:203–209

    Article  CAS  Google Scholar 

  • Zhang F-L, Takahata Y, Watanabe M, Xu J-B (2000) Agrobacterium-mediated transformation of cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep 19:569–575

    Article  Google Scholar 

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Acknowledgement

We thank Dr. R. Aida (National Institute of Floricultural Science, Japan) for the generous gift of ticarcillin and for helpful suggestions.

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Author notes

  1. Yoichi Ogawa

    Present address: NEDO Laboratory of Applied Plant Genomics, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan

Authors and Affiliations

  1. Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba, 271-8510, Japan

    Yoichi Ogawa & Masahiro Mii

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  1. Yoichi Ogawa
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  2. Masahiro Mii
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Correspondence to Masahiro Mii.

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Communicated by J.C. Register III

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Ogawa, Y., Mii, M. Evaluation of 12 β-lactam antibiotics for Agrobacterium-mediated transformation through in planta antibacterial activities and phytotoxicities. Plant Cell Rep 23, 736–743 (2005). https://doi.org/10.1007/s00299-004-0885-9

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  • DOI: https://doi.org/10.1007/s00299-004-0885-9

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