Direct somatic embryogenesis and plant regeneration from leaf, petiole, and stem explants of Golden Pothos

Abstract

Somatic embryos directly formed at cut edges or on the surface of leaf explants, around cut ends or along side surfaces of petiole and stem explants of ‘Golden Pothos’ [Epipremnum aureum (Linden & Andre) Bunt.] on Murashige and Skoog (MS) medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) or N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (TDZ) with α-naphthalene acetic acid (NAA) and a medium called MK containing MS salts with Kao’s vitamins, supplemented with 2.0 mg/l TDZ and 0.2 mg/l NAA. Somatic embryos were also produced on MS medium containing 2.0 mg/l kinetin (KN) and 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) from leaf and petiole explants, MS medium supplemented with 2.0 mg/l CPPU and 0.5 mg/l 2,4-D from petiole and stem explants, and 2.0 mg/l TDZ and 0.2 mg/l or 0.5 mg/l 2,4-D from stem explants. In addition, somatic embryos occurred from stem explants on Chu’s N6 medium containing 2.0 mg/l CPPU and 0.2 mg/l NAA. Somatic embryos matured and grew into multiple buds, shoots, or even plantlets after 2–3 months on the initial culture medium. Germination was optimal on MS medium containing either 2 mg/l 6-benzylaminopurine (BA) and 0.2 mg/l NAA or 2 mg/l zeatin and 0.2 mg/l NAA. Shoots elongated better and roots developed well on MS medium with no growth regulators. Approximately 30–100 plantlets were regenerated from each explant. The regenerated plants grew vigorously after transplanting to a soil-less container substrate in a shaded greenhouse.

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Abbreviations

BA:

6-Benzylaminopurine

CPPU:

N-(2-Chloro-4-pyridyl)-N′-phenylurea

2,4-D:

2,4-Dichlorophenoxyacetic acid

KM:

Kao’s medium

KN:

Kinetin

MES:

2-(N-Morpholino) ethane-sulfonic acid

MS:

Murashige and Skoog’s medium

N6:

Chu’s (N6) medium

NAA:

α-Naphthalene acetic acid

TDZ:

N-Phenyl-N′-1,2,3-thiadiazol-5-ylurea

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Acknowledgements

We thank Dr. Dennis J. Gray for providing photographic equipment used in this study and for his valuable comments on this manuscript’s revision, and Kelly Everitt for critical reading of this manuscript. This research was supported in part by the Gloeckner Foundation, Inc., New York and USDA-TSTAR, and approved for publication as journal series R-10158 by the Florida Agricultural Experiment Station.

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Correspondence to J. Chen.

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Communicated by K.K. Kamo

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Zhang, Q., Chen, J. & Henny, R.J. Direct somatic embryogenesis and plant regeneration from leaf, petiole, and stem explants of Golden Pothos. Plant Cell Rep 23, 587–595 (2005). https://doi.org/10.1007/s00299-004-0882-z

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Keywords

  • CPPU
  • Epipremnum aureum
  • Ornamental foliage plants
  • Pothos
  • Somatic embryogenesis