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Induction of callus from a metal hypertolerant fern, Athyrium yokoscense, and evaluation of its cadmium tolerance and accumulation capacity

  • Biotic and Abiotic Stress
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Abstract

The callus of a metal hypertolerant fern, Athyrium yokoscense, was induced from the spores generated on a small sectioned frond in vitro. The callus grew vigorously with the periodical medium change, especially in a liquid culture. When the callus and regenerated tissues were exposed to Cd, every tissue tolerated at least 1 mM Cd for >1 month. These tissues accumulated high levels of Cd (maximum 3.3 mg g−1 dry weight in roots) in accordance with the Cd concentration of the medium, and the Cd concentrations of all parts, except roots, were at a similar level. The data suggest that the Cd tolerance of this fern is basically independent of the plant parts and the developmental stages, although the accumulation ability is higher in roots than in the other plant parts.

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Acknowledgements

We thank Ms S. Sumino (CRIEPI) for her outstanding technical assistance. This research was supported in part by a research grant from the Core Research for Evolutionary Science and Technology and by a Grant-in-aid for Scientific Research from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Plant Molecular Biology, Laboratory of Environmental Science, Central Research Institute Electric Power Industry, 1646 Abiko, Abiko, Chiba, 270-1194, Japan

    Toshihiro Yoshihara, Kyoko Tsunokawa, Yoshiyuki Miyano, Yuki Arashima, Hirotaka Hodoshima, Kazuhiro Shoji & Fumiyuki Goto

  2. Plant Molecular Biology and Molecular Genetics, Department of Biological Science and Technology, Science University of Tokyo, Yamazaki 2641, Noda, Chiba, 278-8510, Japan

    Yoshiyuki Miyano, Yuki Arashima, Hirotaka Hodoshima & Hiroaki Shimada

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  1. Toshihiro Yoshihara
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  2. Kyoko Tsunokawa
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  3. Yoshiyuki Miyano
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  4. Yuki Arashima
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  5. Hirotaka Hodoshima
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  6. Kazuhiro Shoji
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  8. Fumiyuki Goto
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Correspondence to Toshihiro Yoshihara.

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Communicated by H. Uchimiya

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Yoshihara, T., Tsunokawa, K., Miyano, Y. et al. Induction of callus from a metal hypertolerant fern, Athyrium yokoscense, and evaluation of its cadmium tolerance and accumulation capacity. Plant Cell Rep 23, 579–585 (2005). https://doi.org/10.1007/s00299-004-0877-9

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  • DOI: https://doi.org/10.1007/s00299-004-0877-9

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