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Glycoalkaloid aglycone accumulations associated with infection by Clavibacter michiganensis ssp. sepedonicus in potato species Solanum acaule and Solanum tuberosum and their interspecific somatic hybrids

  • Genetic Transformation and Hybridization
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Abstract

Solanum acaule Bitt., a wild potato species, is closely related to cultivated potato (Solanum. tuberosum L.). Incorporation of desirable traits from allotetraploid [2n=4x=48, 2 endosperm balance number (EBN)] S. acaule (acl) into autotetraploid (2n=4x=48, 4EBN) S. tuberosum (tbr) is difficult due to incongruity boundaries. In this study, three hybrid combinations, each with a specific genome constitution, were produced through protoplast fusion: (1) hexaploid 2x acl (+) 4x tbr, (2) tetraploid 2x acl (+) 2x tbr, and (3) hexaploid 4x acl (+) 2x tbr hybrids. In terms of glycoalkaloid aglycones, the hybrids produced demissidine, tomatidine and solanidine, similarly to the S. acaule parental species, but S. tuberosum synthesised only solanidine. Inoculations with Clavibacter michiganensis ssp. sepedonicus (Cms), which is the causal agent of bacterial ring rot in potato, yielded significantly lower total glycoalkaloid aglycone accumulation both in S. acaule plants and in interspecific hybrids in comparison with the corresponding mock-inoculated plants. However, in S. tuberosum the aglycone levels were either higher or unchanged as a result of infection by Cms. To incorporate the desirable traits of the interspecific somatic hybrids into 4EBN S. tuberosum, sexual backcrosses were carried out. The hexaploid 4x acl (+) 2x tbr hybrids with the hypothetical 4EBN showed the greatest capacity to undergo backcrosses with S. tuberosum.

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Acknowledgements

The authors wish to acknowledge Ms. Tiina Marttila (protoplast fusion), Ms. Sisko Holm (PCR analyses), Ms. Marja-Leena Manninen (glycoalkaloid aglycone extraction) and Ms. Kirsti Mäkelä (anther culture) for excellent technical assistance during the process. This work was financially supported by the Finnish Ministry of Agriculture and Forestry, Jenny and Antti Wihuri Foundation, Finnish Cultural Foundation, and the Foundation of Kemira. The authors are also grateful to Docent Jonathan Robinson for critical review of the manuscript.

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Author notes

  1. A. Tauriainen

    Present address: Department of Biological and Environmental Sciences, Plant Biology, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland

Authors and Affiliations

  1. MTT Agrifood Research Finland, Plant Production Research, Crops and Biotechnology, Myllytie 10, 31600, Jokioinen, Finland

    V-M. Rokka, J. Laurila, A. Tauriainen & J. Larkka

  2. Department of Pharmacy, Pharmacognosy Division, University of Helsinki, P.O. Box 55, 00014, Helsinki, Finland

    I. Laakso

  3. Department of Biosciences, University of Helsinki, P.O. Box 56, Viikinkaari 9, 00014, Helsinki, Finland

    M. Metzler

  4. Boreal Plant Breeding Ltd, Myllytie 10, 31600, Jokioinen, Finland

    L. Pietilä

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  1. V-M. Rokka
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  2. J. Laurila
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Correspondence to V-M. Rokka.

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Communicated by D. Dudits

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Rokka, VM., Laurila, J., Tauriainen, A. et al. Glycoalkaloid aglycone accumulations associated with infection by Clavibacter michiganensis ssp. sepedonicus in potato species Solanum acaule and Solanum tuberosum and their interspecific somatic hybrids. Plant Cell Rep 23, 683–691 (2005). https://doi.org/10.1007/s00299-004-0868-x

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  • DOI: https://doi.org/10.1007/s00299-004-0868-x

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