Skip to main content
SpringerLink
Log in

Multiple spindles and cellularization during microsporogenesis in an artificially induced tetraploid accession of Brachiaria ruziziensis (Gramineae)

  • Cell Biology and Morphogenesis
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

The genus Brachiaria is characterized by a majority of polyploid accessions—mainly tetraploid—and apomictic reproduction. Sexuality is found among diploids. To overcome incompatibility barriers, accessions with the same ploidy level are necessarily used in hybridization. Thus, sexual diploid accessions were tetraploidized to be used as female genitors. This paper reports microsporogenesis in an artificially induced tetraploid accession of Brachiaria ruziziensis. Chromosome pairing at diakinesis ranged from univalents to tetravalents, with predominance of bivalents. Irregular chromosome segregation was frequent in both meiotic divisions. During the first division, multiple spindles showing different arrangements were recorded. The spindle position determined the plane of first cytokinesis and the number of chromosomes determined the size of the cell. Meiotic products were characterized by polyads with spores of different sizes. Pollen sterility was estimated at 61.38%. The limitations of using this accession in the breeding program are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–o
Fig. 2a–I

Similar content being viewed by others

References

  • Brown RC, Lemmon BE (1989) Minispindles and cytoplasmic domains in microsporogenesis in orchids. Protoplasma 148:26–32

    Google Scholar 

  • Brown RC, Lemmon BE (1991) The cytokinetic apparatus in meiosis: control of division plane in the absence of a preprophase band of microtubules. In: Lloyd C (ed) The cytoskeletal basis of plant growth and form. Academic, London, pp 259–273

    Google Scholar 

  • Brown RC, Lemmon BE (1992a) Cytoplasmic domain: a model for spatial control of cytokinesis in reproductive cells of plants. EMSA Bull 22:48–53

    Google Scholar 

  • Brown RC, Lemmon BE (1992b) Control of division plane in normal and griseofulvin-treated microsporocytes of Magnolia. J Cell Sci 103:1031–1038

    CAS  Google Scholar 

  • Brown RC, Lemmon BE (1997) Transition from mitotic apparatus to cytokinetic apparatus in pollen mitosis of the slipper orchid. Protoplasma 198:43–52

    Google Scholar 

  • Carapetian J, Rupert EA (1977) Meiotic irregularities caused by interacting sterility genes in cultivated safflower (Carthamus tinctorius). Can J Genet Cytol 19:103–109

    Google Scholar 

  • Corazza-Nunes MJ, Pagliarini MS, Silva IS (1993) Polyploidy and its consequences in Aloysia lycioides (Verbenaceae). Arq Biol Tecnol 36:753–759

    Google Scholar 

  • Endow SA (1999) Microtubule motors in spindle and chromosome motility. Eur J Biochem 262:12–18

    Article  CAS  PubMed  Google Scholar 

  • Ferguson JE, Crowder LV (1974) Cytology and breeding behaviour of Brachiaria ruziziensis. Crop Sci 14:893–895

    Google Scholar 

  • Gobbe J, Swenne A, Louant BP (1981) Diploides naturals et autotetraploides induits chez Brachiaria ruziziensis Germain et Evard: critères d’identification. Agron Trop 36:339–346

    Google Scholar 

  • Hatsumi M, Endow SA (1992) The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitotic cells. J Cell Sci 103:1013–1020

    CAS  PubMed  Google Scholar 

  • Mendes-Bonato AB, Pagliarini MS, Valle CB, Penteado MIO (2001) Meiotic instability in invader plants of signal grass Brachiaria decumbens Stapf (Gramineae). Acta Scientiarum 23:619–625

    Google Scholar 

  • Mendes-Bonato AB, Junqueira Filho RG, Pagliarini MS, Valle CB, Penteado MIO (2002) Unusual cytological patterns of microsporogenesis in Brachiaria decumbens: abnormalities in spindle and defective cytokinesis causing precocious cellularization. Cell Biol Int 26:641–646

    Article  PubMed  Google Scholar 

  • Miles JW, Valle CB (1966) Manipulation of apomixis in Brachiaria breeding. In: Miles JW, Maass BL, Valle CB (eds) Brachiaria: biology, agronomy, and improvement. CIAT Publication No. 259. Centro Internacional de Agricultura Tropical—CIAT/Empresa Brasileira de Pesquisa Agropecuária—EMBRAPA, Colombia, pp 164–177

    Google Scholar 

  • Pagliarini MS (1990) Instabilidade meiótica em Thunbergia mysorensis (Acanthaceae). Cienc Cult 42:83–87

    Google Scholar 

  • Penteado MIO, Santos ACM, Rodrigues IF, Valle CB, Seixas MAC, Esteves A (2000) Determinação de ploidia e avaliação da quantidade de DNA total em diferentes espécies do gênero Brachiaria. Campo Grande: Embrapa Gado de Corte, Boletim de Pesquisa No. 11. EMBRAPA Gado de Corte

    Google Scholar 

  • Pinheiro AA, Pozzobon MT, Valle CB, Penteado MIO, Carneiro VTC (2000) Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine. Plant Cell Rep 19:274–278

    Article  CAS  Google Scholar 

  • Risso-Pascotto C, Pagliarini MS, Valle CB, Mendes-Bonato AB (2003a) Chromosome number and microsporogenesis in a pentaploid accession of Brachiaria brizantha (Gramineae). Plant Breed 122:136–140

    Google Scholar 

  • Risso-Pascotto C, Pagliarini MS, Valle CB (2003b) A mutation in the spindle checkpoint arresting meiosis II in Brachiaria ruziziensis. Genome 46:724–728

    Article  PubMed  Google Scholar 

  • Shamina N, Doragova N, Trunova S (2000) Radial spindle and the phenotype of the maize meiotic mutant, dv. Cell Biol Int 24:729–736

    Article  CAS  PubMed  Google Scholar 

  • Singh RJ (1993) Plant cytogenetics. CRC, Boca Raton

    Google Scholar 

  • Staiger CJ, Cande WZ (1990) Microtubule distribution in dv, a maize meiotic mutant defective in the prophase to metaphase transition. Dev Biol 138:213–242

    CAS  PubMed  Google Scholar 

  • Swenne A, Louant BP, Dujardin M (1981) Induction par la colchicine de formes autotétraploides chez Brachiaria ruziziensis Germain et Evard (Gramínée). Agron Trop 36:134–141

    Google Scholar 

  • Tai W (1970) Multipolar meiosis in diploid crested wheat-grass Agropyrum cristatum. Am J Bot 57:1160–1169

    Google Scholar 

  • Thompson MM (1962) Cytogenetics of Rubus. III. Meiotic instability in some higher polyploids. Am J Bot 49:575–582

    Google Scholar 

  • Tilquin JP, Brower K, Howart F (1984) Unusual cytological patterns of microsporogenesis in a cultivar of Fuchsia. 1. Multiple spindles. Theor Appl Genet 67:413–417

    Google Scholar 

  • Valle CB, Miles JM (2001) Breeding apomictic species. In: Savidan Y, Carman JG, Dresselhaus T (eds) The flowering of apomixis: from mechanisms to genetic engineering. CIMMYT, IRD, European Commission DG VI (FAIR), Mexico, pp 137–152

    Google Scholar 

  • Valle CB, Savidan Y (1996) Genetics, cytogenetics, and reproductive biology of Brachiaria. In: Miles JW, Maass BL, Valle CB (eds) Brachiaria: biology, agronomy, and improvement. CIAT Publication No. 259. Centro Internacional de Agricultura Tropical—CIAT/Empresa Brasileira de Pesquisa Agropecuária—EMBRAPA, Colombia, pp 147–163

    Google Scholar 

  • Valle CB, Macedo MCM, Calixto S (2000) Avaliação agronômica de híbridos de Brachiaria. In: Reunião Anual da Sociedade Brasileira de Zootecnia, Anais. Viçosa: SBZ. 2000. ICD-ROM. Oral, Forragicultura, FOR 383

  • Walters MS (1960) Rates of meiosis, spindle irregularities and microsporocyte division in Bromus trinii × B. carinatus. Chromosoma 11:167–204

    CAS  PubMed  Google Scholar 

  • Woods LM, Hodges CA, Baart E, Baker SM, Liskay M, Hun PA (1999) Chromosomal influence on meiotic spindle assembly: abnormal meiosis I in female Mlh1 mutant mice. J Cell Biol 145:1395–1406

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringa, PR, Brazil

    Claudicéia Risso-Pascotto & Maria Suely Pagliarini

  2. Embrapa Beef Cattle, P.O. Box 154, 79002-970, Campo Grande, MS, Brazil

    Cacilda Borges do Valle

Authors
  1. Claudicéia Risso-Pascotto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria Suely Pagliarini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cacilda Borges do Valle
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria Suely Pagliarini.

Additional information

Communicated by P. Debergh

Rights and permissions

Reprints and permissions

About this article

Cite this article

Risso-Pascotto, C., Pagliarini, M.S. & do Valle, C.B. Multiple spindles and cellularization during microsporogenesis in an artificially induced tetraploid accession of Brachiaria ruziziensis (Gramineae). Plant Cell Rep 23, 522–527 (2005). https://doi.org/10.1007/s00299-004-0867-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-004-0867-y

Keywords

Search

Navigation