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A variable region of the Sugarcane Bacilliform Virus (SCBV) genome can be used to generate promoters for transgene expression in sugarcane

  • Genetic Transformation and Hybridization
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Abstract

Four promoters derived from sugarcane bacilliform virus (SCBV) were compared and characterised. Three were obtained by PCR amplification of purified virion DNA extracted from three sugarcane cultivars. The fourth promoter was obtained by subcloning from an almost genome-length clone of SCBV. All promoters were able to drive stable expression of β-glucuronidase in sugarcane. The PCR-derived promoter sequences shared more DNA homology with banana streak virus than to the subcloned SCBV. The subcloned promoter was the strongest expressing and was able to drive reporter gene expression in vitro and in the leaves, meristems and roots of glasshouse-grown sugarcane. Expression levels were at least equal to or higher than those measured for the maize polyubiquitin promoter.

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Acknowledgements

This work was funded by BSES Limited. We wish to thank Andrew Geering and John Thomas for access to BSV sequences, Ben Lockhart and Neil Olszweski for providing pSCBV20, Del Greenway for the statistical analyses and Warren Owens for providing sugarcane material.

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Author notes

  1. G. R. Smith

    Present address: New Zealand Institute for Crop and Food Research Limited, Private Bag 4704, Christchurch, New Zealand

Authors and Affiliations

  1. David North Plant Research Centre, BSES Limited, P.O. Box 86, Indooroopilly, Queensland, 4068, Australia

    K. S. Braithwaite, R. J. Geijskes & G. R. Smith

Authors
  1. K. S. Braithwaite
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  2. R. J. Geijskes
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  3. G. R. Smith
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Correspondence to K. S. Braithwaite.

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Communicated by P. Lakshmanan

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Braithwaite, K.S., Geijskes, R.J. & Smith, G.R. A variable region of the Sugarcane Bacilliform Virus (SCBV) genome can be used to generate promoters for transgene expression in sugarcane. Plant Cell Rep 23, 319–326 (2004). https://doi.org/10.1007/s00299-004-0817-8

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  • DOI: https://doi.org/10.1007/s00299-004-0817-8

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