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Adventitious root formation in Arabidopsis thaliana thin cell layers

  • Cell Biology and Morphogenesis
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Abstract

This paper describes, for the first time, de novo adventitious root formation from thin cell layers (TCLs) of Arabidopsis thaliana. The objective of the study was to determine the optimal hormonal and light conditions and the optimal exogenous Ca2+ concentration for obtaining adventitious rooting (AR) from A. thaliana TCLs and to identify the tissue(s) involved in the process. The results show that maximum AR was obtained with a single-phase method in the presence of 10 μM indole-3-butyric acid and 0.1 μM kinetin under continuous darkness for 30 days and with 0.6 mM exogenous CaCl2. The endodermis was the only tissue involved in root meristemoid formation. The role of Ca2+ in AR and the importance of using Arabidopsis TCLs in studies on the genetic/biochemical control of AR are discussed.

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Fig. 1a–f
Fig. 2a–m
Fig. 3a–k

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Abbreviations

AR :

Adventitious rooting

CIM :

Callus-inducing medium

Col-0 :

Columbia ecotype

2,4-D :

2,4-Dichlorophenoxyacetic acid

HFM :

Hormone-free medium

HM :

Medium with 10 μM IBA and 0.1 μM Kin

IBA :

Indole-3-butyric acid

Kin :

Kinetin

LS :

Longitudinal section

NAA :

α-Naphthaleneacetic acid

RIM :

Root-inducing medium

TCL :

Thin cell layer

WS :

Wassilewskija ecotype

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Acknowledgements

This work was supported by Università La Sapienza (Rome, Italy), Progetto Ateneo, to M.M.A. The authors express their gratitude to Marisa Tomassi for support in standardizing the Arabidopsis TCL technique, and to Stefania Biondi for helpful discussions.

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Authors and Affiliations

  1. Dipartimento di Biologia Vegetale, University “La Sapienza”, P. le Aldo Moro, 5, 00185, Rome, Italy

    G. Falasca, D. Zaghi & M. M. Altamura

  2. Unità di Nutrizione Sperimentale, Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione, Rome, Italy

    M. Possenti

Authors
  1. G. Falasca
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  2. D. Zaghi
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  3. M. Possenti
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  4. M. M. Altamura
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Correspondence to M. M. Altamura.

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Communicated by A. Altman

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Falasca, G., Zaghi, D., Possenti, M. et al. Adventitious root formation in Arabidopsis thaliana thin cell layers. Plant Cell Rep 23, 17–25 (2004). https://doi.org/10.1007/s00299-004-0801-3

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  • DOI: https://doi.org/10.1007/s00299-004-0801-3

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