Abstract
The mechanism of nuclear export of RNAs in yeast and animal cells is rapidly being uncovered, but RNA export in plants has received little attention. We introduced capped and uncapped fluorescent mRNAs into tobacco (Nicotiana plumbaginifolia) protoplasts and studied their cellular localization. Following insertion, capped transcripts were found in the cytoplasm, while uncapped messengers transiently appeared in the nucleus in about one-quarter to one-third of the cells. These mRNAs were trapped by the nuclear export-inhibiting drug leptomycin B, pointing to an export mechanism in plants similar to Rev-NES-mediated RNP export in other organisms.
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Acknowledgements
We thank Minoru Yoshida for the generous gift of leptomycin B before it became commercially available; Sigrid Ranostaj and other colleagues for technical assistance; Daniela Wagner, Frank Landgraf, Dudley Page, and Hendrik Raué for critical reading of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (DFG).
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Stuger, R., Forreiter, C. Uncapped mRNA introduced into tobacco protoplasts can be imported into the nucleus and is trapped by leptomycin B. Plant Cell Rep 23, 99–103 (2004). https://doi.org/10.1007/s00299-004-0780-4
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DOI: https://doi.org/10.1007/s00299-004-0780-4