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Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and ‘Morita navel’ orange (Citrus sinensis Osbeck)

  • Genetic Transformation and Hybridization
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Abstract

Intergeneric somatic hybrids between embryogenic callus-derived protoplasts of round kumquat (Fortunella japonica Swingle) and ‘Morita navel’ orange (Citrus sinensis Osbeck) were produced by electrofusion. Among the eight different fusion strains obtained, six showed normal morphology, whereas the remaining two showed malformation. All the regenerated plants were intermediate in leaf morphology and had thick and round leaves, which are typical characteristics of polyploids. Ploidy analyses by flow cytometry and chromosome counting in root-tip cells revealed that these plants are amphidiploid (2n=4×=36). Hybridity of the fusion products was confirmed by random amplified polymorphic DNA and cleaved amplified polymorphic sequence (CAPS) analyses. Furthermore, analyses of chloroplast (cp) and mitochondrial (mt) DNA by CAPS showed that these somatic hybrids contained cp- and mt-DNA of round kumquat without recombination in the regions analyzed.

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Abbreviations

BA :

6-Benzylaminopurine

CAPS :

Cleaved amplified polymorphic sequence

RAPD :

Random amplified polymorphic DNA

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Acknowledgements

The authors are grateful to Dr. Masahiro Mii of the Faculty of Horticulture, Chiba University, for his advice and critical reading of this manuscript. This work was supported in part by a grant-in-aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

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Authors and Affiliations

  1. Faculty of Agriculture, Miyazaki University, Gakuenkibanadai, 889-2192, Miyazaki , Japan

    K. Takami, T. Imayama & H. Kunitake

  2. School of Agriculture, Kyusyu Tokai University, Choyo, Aso, 869-1404, Kumamoto , Japan

    A. Matsumara, M. Yahata & H. Komatsu

Authors
  1. K. Takami
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  2. A. Matsumara
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  3. M. Yahata
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  4. T. Imayama
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  5. H. Kunitake
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  6. H. Komatsu
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Correspondence to H. Kunitake.

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Communicated by H. Ebinuma

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Takami, K., Matsumara, A., Yahata, M. et al. Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and ‘Morita navel’ orange (Citrus sinensis Osbeck). Plant Cell Rep 23, 39–45 (2004). https://doi.org/10.1007/s00299-004-0777-z

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  • DOI: https://doi.org/10.1007/s00299-004-0777-z

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